Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture and Rural Affairs, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, 100081, People's Republic of China.
Shanghai Academy of Landscape Architecture Science and Planning, Shanghai, 200232, People's Republic of China.
Curr Microbiol. 2022 Aug 27;79(10):302. doi: 10.1007/s00284-022-02995-2.
A Gram-positive, endospore-forming, rod-shaped and aerobic bacterium, with swarming and swimming motility, designated strain DBTR6, was isolated from the rhizosphere soil of tomato plants. Strain DBTR6 grew at 20-45 ℃ (optimum 30-37℃), pH 4-9 (optimum 7-8) and at salinities from 0 to 5% (optimum 1%). Phylogenetic analysis using 16S rRNA gene sequences showed this strain belonged to the genus Metabacillus and was most closely related to Metabacillus litoralis DSM 16303 (98.3%) and Metabacillus sediminilitoris MCCC 1K03777 (98.3%). The DNA G + C content of the genomic DNA was 36.4%. The digital DNA-DNA hybridization value between strain DBTR6 and reference strains M. sediminilitoris MCCC 1K03777 and "M. bambusae" BG109 were less than 70% (26.7% and 26.0%), and the average nucleotide identity score were less than 95% (78.55% and 78.38%), and the Amino Acid Identity values calculated were less than 96% (79.99% and 80.18%), respectively, suggesting that strain DBTR6 represented a novel species in the genus Metabacillus. Chemotaxonomic analysis showed that strain DBTR6 contained MK-7 as the major respiratory quinone. The predominant fatty acids (> 10.0%) were iso-C, anteiso-C and C. The major polar lipids were diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), glycolipids (GL) and three unidentified lipids (L). Based on the differential physiological properties, biochemical characteristics and genotypic data, strain DBTR6 represents a novel species of the genus Metabacillus, for which the name Metabacillus rhizolycopersici sp. nov. is proposed. The type strain is DBTR6 (= ACCC 61900 = JCM 35080).
一株革兰氏阳性、产芽孢、杆状、需氧细菌，具有群集和游动运动性，被命名为 DBTR6 菌株，从番茄植物根际土壤中分离得到。DBTR6 菌株在 20-45°C（最佳 30-37°C）、pH 值 4-9（最佳 7-8）和从 0 到 5%的盐度（最佳 1%）下生长。使用 16S rRNA 基因序列的系统发育分析表明，该菌株属于代谢芽孢杆菌属，与 litoralis DSM 16303（98.3%）和 sediminilitoris MCCC 1K03777（98.3%）最为密切相关。基因组 DNA 的 G+C 含量为 36.4%。菌株 DBTR6 与参考菌株 M. sediminilitoris MCCC 1K03777 和“M. bambusae”BG109 的数字 DNA-DNA 杂交值均小于 70%（分别为 26.7%和 26.0%），平均核苷酸同一性得分均小于 95%（分别为 78.55%和 78.38%），计算的氨基酸同一性值均小于 96%（分别为 79.99%和 80.18%），表明菌株 DBTR6 代表代谢芽孢杆菌属中的一个新种。化学分类分析表明，菌株 DBTR6 含有 MK-7 作为主要呼吸醌。主要脂肪酸（>10.0%）为 iso-C、anteiso-C 和 C。主要极性脂为二磷脂酰甘油（DPG）、磷脂酰乙醇胺（PE）、磷脂酰甘油（PG）、糖脂（GL）和三种未鉴定的脂（L）。基于差异生理特性、生化特征和基因型数据，菌株 DBTR6 代表代谢芽孢杆菌属的一个新种，命名为根际解淀粉芽孢杆菌。模式菌株为 DBTR6（=ACCC 61900=JCM 35080）。
