Sourek J, Trnka T, Levin J, Roubal J, Michalec C
Infect Immun. 1979 Feb;23(2):465-71. doi: 10.1128/iai.23.2.465-471.1979.
Mild acetic acid hydrolysis of endotoxin (lipopolysaccharide-protein complex) of Shigella dysenteriae type 1 (S and R forms) yielded a lipid A-protein complex that consisted of amino acids, fatty acids, and sugar and, in terms of chemical composition, displayed no marked differences between the S and R forms. Its protein portion (53 to 56%) consisted of at least 16 amino acids. In the fatty acid portion (14 to 18%), myristic, 3-hydroxymyristic, palmitic, and stearic acids accounted for 50%. The sugar portion (10 to 12%) consisted solely of glucosamine. The remainder was unidentified substances, most of which contained phosphorus. Lipid A-protein complexes derived from both S and R forms were not toxic for mice in doses up to 1,000 microgram/mouse, but their Linulus test activity had increased considerably as compared with the starting lipopolysaccharide-protein complex material: from 10(-6) to 10(-10--10(-12) mg/ml. The lipid A-protein complexes were readily soluble in a water solution of triethylamine, in dimethyl sulfoxide, and in pyridine.
对1型痢疾志贺氏菌（S型和R型）的内毒素（脂多糖 - 蛋白质复合物）进行轻度乙酸水解，得到一种脂多糖 - 蛋白质复合物，其由氨基酸、脂肪酸和糖类组成，就化学组成而言，S型和R型之间没有明显差异。其蛋白质部分（53%至56%）至少由16种氨基酸组成。在脂肪酸部分（14%至18%）中，肉豆蔻酸、3 - 羟基肉豆蔻酸、棕榈酸和硬脂酸占50%。糖类部分（10%至12%）仅由氨基葡萄糖组成。其余为未鉴定物质，其中大部分含有磷。从S型和R型衍生而来的脂多糖 - 蛋白质复合物，剂量高达1000微克/小鼠时对小鼠无毒，但与起始的脂多糖 - 蛋白质复合物材料相比，其鲎试剂检测活性显著增加：从10^(-6)提高到10^(-10) - 10^(-12)毫克/毫升。脂多糖 - 蛋白质复合物易溶于三乙胺水溶液、二甲基亚砜和吡啶。
