Limjuco G A, Karkhanis Y D, Zeltner J Y, Maigetter R Z, King J J, Carlo D J
J Gen Microbiol. 1978 Feb;104(2):187-91. doi: 10.1099/00221287-104-2-187.
A lipopolysaccharide was isolated from Neisseria meningitidis group B by phenol/water extraction and purified by differential ultracentrifugation. This preparation exhibited endotoxic properties as shown by the limulus-lysate assay. Mild acid hydrolysis of the lipopolysaccharides yielded a lipid A fraction and a polysaccharide fraction. The lipid A fraction contained fatty acids, phosphorus and glucosamine. Analysis of the polysaccharide fraction revealed the presence of glucose, galactose, glucosamine, 2-keto-3-deoxyoctonic acid and phosphorus. There was no heptose.
通过苯酚/水萃取法从B群脑膜炎奈瑟菌中分离出一种脂多糖，并通过差速超速离心法进行纯化。如鲎试剂检测所示，该制剂具有内毒素特性。脂多糖经温和酸水解后产生脂质A部分和多糖部分。脂质A部分含有脂肪酸、磷和氨基葡萄糖。对多糖部分的分析显示存在葡萄糖、半乳糖、氨基葡萄糖、2-酮-3-脱氧辛酸和磷。没有庚糖。
