Department of Microbiology and Immunology, Sidney Kimmel Medical College, Thomas Jefferson University, Philadelphia, PA, 19107, USA; Sidney Kimmel Cancer Center, Jefferson Health, Philadelphia, PA, 19107, USA.
Department of Microbiology and Immunology, Sidney Kimmel Medical College, Thomas Jefferson University, Philadelphia, PA, 19107, USA; Sidney Kimmel Cancer Center, Jefferson Health, Philadelphia, PA, 19107, USA.
Cryobiology. 2023 Jun;111:142-145. doi: 10.1016/j.cryobiol.2023.03.005. Epub 2023 Mar 29.
The CryoPreservation Media (CPM) for mouse sperm using raffinose and skim milk have been improved by adding either monothioglycerol (MTG) or l-glutamine to reduce the oxidative damage during sperm freezing and thawing. The CARD-CPM utilizing l-glutamine, but not MTG, has been widely used to meet the rising demand for cryopreservation of genetically modified mice, as the CARD method also improved sperm capacitation and in vitro fertilization (IVF). However, the viability of sperm frozen in the CARD-CPM is highly variable, indicating a room for improvement. To develop a more dependable technique for mouse sperm cryopreservation, we investigate whether combining MTG and l-glutamine in the CPM (MG-CPM) can produce a synergistic impact on sperm thawing and IVF rate. We found that MG-CPM reduced the incidence of infertility and increased the IVF success rate. Therefore, cryopreservation of mouse sperm in MG-CPM is a reliable method to ensure embryo generation from frozen sperm.
使用棉子糖和脱脂乳的冷冻保护剂（CPM）可通过添加单硫甘油（MTG）或 L-谷氨酰胺来改善，以减少精子冷冻和解冻过程中的氧化损伤。利用 L-谷氨酰胺的 CARD-CPM，但不是 MTG，已被广泛用于满足对基因修饰小鼠冷冻保存的需求增加，因为 CARD 方法还改善了精子获能和体外受精（IVF）。然而，在 CARD-CPM 中冷冻的精子的活力变化很大，表明仍有改进的空间。为了开发更可靠的小鼠精子冷冻保存技术，我们研究了在 CPM 中（MG-CPM）结合 MTG 和 L-谷氨酰胺是否对精子解冻和 IVF 率产生协同影响。我们发现 MG-CPM 降低了不育症的发生率并提高了 IVF 成功率。因此，在 MG-CPM 中冷冻保存小鼠精子是一种可靠的方法，可确保从冷冻精子中生成胚胎。
