State Key Laboratory of Animal Biotech Breeding and Key Laboratory of Soil Microbiology, Ministry of Agriculture, College of Biological Sciences, China Agricultural University, Beijing, China; Center for Advanced Measurement Science, National Institute of Metrology, Beijing, China.
State Key Laboratory of Animal Biotech Breeding and Key Laboratory of Soil Microbiology, Ministry of Agriculture, College of Biological Sciences, China Agricultural University, Beijing, China.
Bioresour Technol. 2023 Jun;378:128990. doi: 10.1016/j.biortech.2023.128990. Epub 2023 Mar 30.
In order to optimize the composition of enzyme cocktail for improving the hydrolytic efficiency of lignocellulose, different substrates were tested as inducers for producing lignocellulolytic enzymes by Trichoderma harzianum EM0925 in this study. As results, ultrafine grinding or steam explosion pretreated substrates can induce T. harzianum EM0925 to secret holo lignocellulolytic enzymes; acid treated substrate can induce cellobiohydrolase; while alkali or sodium chlorite treated substrates can induce β-xylosidase specifically. Furthermore, the combination of enzyme cocktails with different hydrolysis characteristics can further improve the hydrolysis efficiency, since 100% yields of glucose and xylose were obtained simultaneously from ultrafine grinding treated corn stover at low enzyme dosage (1.2 mg proteins/g substrate). This study for the first time demonstrated an effective solution that specific-pretreated substrates can be used as inducers for specific enzyme production by T. harzianum, which provided new idea and potential strategy for the construction of highly-efficient lignocellulolytic enzyme cocktails.
为了优化酶混合物的组成以提高木质纤维素的水解效率，本研究采用哈茨木霉 EM0925 作为产木质纤维素酶的诱导物，测试了不同的底物。结果表明，超细粉碎或蒸汽爆破预处理的底物可以诱导哈茨木霉 EM0925 分泌完整的木质纤维素酶；酸处理的底物可以诱导纤维二糖水解酶；而碱或亚氯酸钠处理的底物可以特异性诱导β-木糖苷酶。此外，不同水解特性的酶混合物的组合可以进一步提高水解效率，因为在低酶用量（1.2 mg 蛋白质/g 底物）下，从超细粉碎处理的玉米秸秆中可以同时获得 100%的葡萄糖和木糖收率。本研究首次证明了一种有效的解决方案，即特定预处理的底物可以作为哈茨木霉特定酶产生的诱导物，为构建高效木质纤维素酶混合物提供了新的思路和潜在策略。
