Key Laboratory of Horticulture Science for Southern Mountains Regions of Ministry of Education, College of Horticulture and Landscape Architecture, Southwest University, Chongqing, China.
Genes Genomics. 2023 Jul;45(7):921-934. doi: 10.1007/s13258-023-01375-9. Epub 2023 Apr 1.
The plant-specific valine-glutamine (VQ) motif containing proteins tightly regulate plant growth, development, and stress responses. However, the genome-wide identification and functional analysis of Brassica oleracea (B. oleracea) VQ genes have not been reported.
To identify the VQ gene family in B. oleracea and analyze the function of Bo25-1 in pollen germination.
The Hidden Markov Model (HMM) of VQ family was used to query the BoVQ genes in the B. oleracea genome. The BoVQ genes preferentially expressed in anthers were screened by qRT-PCR. Subcellular localization of VQ25-1 was observed in Nicotiana benthamiana (N. benthamiana) leaves. To analysis the role of BoVQ25-1 in pollen germination, the expression of BoVQ25-1 was suppressed using antisense-oligonucleotides (AS-ODN).
A total of 64 BoVQ genes were identified in the B. oleracea genome. BoVQ25-1 was found to be preferentially expressed in the B. oleracea anthers. BoVQ25-1 was cloned from the anthers of the B. oleracea cultivar 'Fast Cycle'. BoVQ25-1 is localized to the nucleus. The pollen germination rate significantly decreased after AS-ODN treatment.
Sixty-four BoVQ genes were identified in the B. oleracea genome, of which BoVQ25-1 plays an important role in pollen germination.
植物特有的缬氨酸-谷氨酰胺（VQ）基序包含的蛋白质严格调控植物的生长、发育和应激反应。然而，甘蓝型油菜（B.oleracea）VQ 基因的全基因组鉴定和功能分析尚未报道。
鉴定甘蓝型油菜中的 VQ 基因家族，并分析 Bo25-1 在花粉萌发中的功能。
使用 VQ 家族的隐马尔可夫模型（HMM）在甘蓝型油菜基因组中查询 BoVQ 基因。通过 qRT-PCR 筛选在花药中优先表达的 BoVQ 基因。观察 VQ25-1 在烟草原生质体中的亚细胞定位。为了分析 BoVQ25-1 在花粉萌发中的作用，使用反义寡核苷酸（AS-ODN）抑制 BoVQ25-1 的表达。
在甘蓝型油菜基因组中鉴定出 64 个 BoVQ 基因。发现 BoVQ25-1 在甘蓝型油菜花药中优先表达。从甘蓝型油菜品种‘Fast Cycle’的花药中克隆出 BoVQ25-1。BoVQ25-1 定位于细胞核。AS-ODN 处理后花粉萌发率显著降低。
在甘蓝型油菜基因组中鉴定出 64 个 BoVQ 基因，其中 BoVQ25-1 在花粉萌发中起重要作用。
