Meijers J C, Lim C K, Lawson A M, Peters T J
J Chromatogr. 1986 Feb 21;352:231-9. doi: 10.1016/s0021-9673(01)83382-3.
Reversed-phase chromatography using a MOS-Hypersil (C8) column with methanol-1 M ammonium acetate buffer (pH 4.6) (60:40) as mobile phase has been developed for the isolation of tumour-localizing haematoporphyrin derivative (HPD). The system effectively resolved the diastereoisomers of haematoporphyrin and its acetyl derivatives. The chromatography peaks were identified by fast-atom bombardment mass spectrometry and were confirmed by chemical synthesis. The main components of HPD before alkaline hydrolysis were diacetylhaematoporphyrin, 8-(1-acetoxyethyl)haematoporphyrin and 3-(1-acetoxyethyl)haematoporphyrin with small amounts of haematoporphyrin, 8-(1-hydroxyethyl)-3-vinyldeuteroporphyrin, 3-(1-hydroxyethyl)-8-vinyldeuteroporphyrin, 8-(1-acetoxyethyl)-3-vinyldeuteroporphyrin, 3-(1-acetoxyethyl)-8-vinyldeuteroporphyrin and protoporphyrin. After hydrolysis with 0.1 M sodium hydroxide, the main components were haematoporphyrin, hydroxyethylvinyldeuteroporphyrins, and protoporphyrin.
已开发出一种反相色谱法，使用MOS-Hypersil（C8）柱，以甲醇 - 1M醋酸铵缓冲液（pH 4.6）（60:40）作为流动相，用于分离肿瘤定位血卟啉衍生物（HPD）。该系统有效地分离了血卟啉及其乙酰衍生物的非对映异构体。通过快原子轰击质谱法鉴定了色谱峰，并通过化学合成进行了确认。碱性水解前HPD的主要成分是二乙酰血卟啉、8-(1-乙酰氧基乙基)血卟啉和3-(1-乙酰氧基乙基)血卟啉，还有少量的血卟啉、8-(1-羟乙基)-3-乙烯基去氢卟啉、3-(1-羟乙基)-8-乙烯基去氢卟啉、8-(1-乙酰氧基乙基)-3-乙烯基去氢卟啉、3-(1-乙酰氧基乙基)-8-乙烯基去氢卟啉和原卟啉。用0.1M氢氧化钠水解后，主要成分是血卟啉、羟乙基乙烯基去氢卟啉和原卟啉。
