Stubbs C, Haigh J M, Kanfer I
J Chromatogr. 1986 Feb 26;353:33-8. doi: 10.1016/s0021-9673(01)87074-6.
The determination of oleandomycin in serum and urine by high-performance liquid chromatography using erythromycin as internal standard is described. The separation was achieved on a reversed-phase C18 column employing acetonitrile-0.05 M phosphate buffer (30:70), adjusted to pH 7.0, as the mobile phase with UV detection at 200 nm. A solid-phase extraction procedure, combined with a simple phase-separation step was used prior to chromatographic analysis. Linear calibration curves were obtained in the concentration ranges 0.25-5.0 micrograms/ml (serum) and 1.0-25.0 micrograms/ml (urine). Precise quantitative analysis has been achieved at these levels with relative standard deviations of less than 5%.
本文描述了以红霉素为内标，采用高效液相色谱法测定血清和尿液中竹桃霉素的方法。分离在反相C18柱上进行，以乙腈-0.05M磷酸盐缓冲液（30:70）（pH调至7.0）作为流动相，在200nm处进行紫外检测。在色谱分析之前，采用了固相萃取程序并结合简单的相分离步骤。在0.25-5.0微克/毫升（血清）和1.0-25.0微克/毫升（尿液）的浓度范围内获得了线性校准曲线。在这些水平上实现了精确的定量分析，相对标准偏差小于5%。
