Stubbs C, Haigh J M, Kanfer I
J Pharm Sci. 1985 Oct;74(10):1126-8. doi: 10.1002/jps.2600741027.
A high-performance liquid chromatographic analysis of erythromycin in human serum and urine with UV detection at 200 nm is presented. The method involves a solid-phase extraction procedure followed by a simple phase separation step and chromatography on a reversed-phase column. The method has sensitivity limits of 0.25 and 1.0 micrograms/mL in serum and urine, respectively, and is sufficiently sensitive to monitor concentrations of erythromycin in human serum and urine after the administration of a single 500-mg erythromycin stearate tablet.
本文介绍了一种采用紫外检测（200nm）对人血清和尿液中的红霉素进行高效液相色谱分析的方法。该方法包括固相萃取步骤，随后是简单的相分离步骤以及在反相柱上进行色谱分析。该方法在血清和尿液中的检测限分别为0.25微克/毫升和1.0微克/毫升，对单次服用500毫克硬脂酸红霉素片后人血清和尿液中红霉素浓度的监测具有足够的灵敏度。
