液相色谱-四极杆飞行时间质谱法（LC-Q-TOF-MS）测定大麻中的11种大麻素

[Determination of 11 Cannabinoids in Cannabis sativa L. by Liquid Chromatography Quadrupole Time-of-Flight Mass Spectrometry (LC-Q-TOF-MS)].

作者信息

Tanaka Rie, Mizutani Sakumi, Kawamura Maiko, Fuchino Hiroyuki, Kawahara Nobuo, Kikura-Hanajiri Ruri

机构信息

Division of Pharmacognosy, Phytochemistry and Narcotics, National Institute of Health Sciences.

Research Center for Medicinal Plant Resources, National Institutes of Biomedical Innovation, Health and Nutrition.

出版信息

Yakugaku Zasshi. 2023;143(4):411-418. doi: 10.1248/yakushi.22-00198.

DOI:10.1248/yakushi.22-00198

PMID:37005243

Abstract

Eleven major cannabinoids from each subdivided tissue of drug-type and fiber-type cannabis plants were determined by means of a liquid chromatography quadrupole time-of-flight mass spectrometry (LC-Q-TOF-MS). The cannabinoids analyzed in this study were tetrahydrocannabinol acid (THCA), Δ-tetrahydrocannabinol (Δ-THC), cannabidiol acid (CBDA), cannabidiol (CBD), Δ-tetrahydrocannabinol (Δ-THC), cannabinol (CBN), cannabichromene (CBC), cannabidivarin (CBDV), cannabigerolic acid (CBGA), cannabigerol (CBG) and tetrahydrocannabivarin (THCV). As a result, THCA was detected in the bracts at 28.4 µg/mg, in the buds at 24.8 µg/mg, and in the leaves at 5.1 to 10.5 µg/mg in the drug-type cannabis plant. In addition, Δ-THC, CBGA, CBN, CBG, CBC, and THCV were mainly detected in bracts, buds, and leaves. On the other hand, as for the fiber-type cannabis plant, CBDA was detected in the bracts at 27.5 µg/mg, in the buds at 10.6 µg/mg, and in the leaves at 1.5-3.3 µg/mg. In addition, Δ-THCA, CBD, Δ-THC, CBC, and CBG were mainly detected in bracts, buds, and leaves.

摘要

采用液相色谱-四极杆飞行时间质谱法（LC-Q-TOF-MS）测定了药用型和纤维型大麻植物各细分组织中的11种主要大麻素。本研究分析的大麻素包括四氢大麻酚酸（THCA）、Δ-四氢大麻酚（Δ-THC）、大麻二酚酸（CBDA）、大麻二酚（CBD）、Δ-四氢大麻酚（Δ-THC）、大麻酚（CBN）、大麻色烯（CBC）、大麻二萜酚（CBDV）、大麻萜酚酸（CBGA）、大麻萜酚（CBG）和四氢大麻萜酚（THCV）。结果表明，在药用型大麻植物中，苞片、芽和叶中均检测到THCA，其含量分别为28.4μg/mg、24.8μg/mg和5.1至10.5μg/mg。此外，Δ-THC、CBGA、CBN、CBG、CBC和THCV主要在苞片、芽和叶中检测到。另一方面，对于纤维型大麻植物，苞片、芽和叶中均检测到CBDA含量分别为27.5μg/mg、10.6μg/mg和1.5 - 3.3μg/mg。此外，Δ-THCA、CBD、Δ-THC、CBC和CBG主要在苞片、芽和叶中检测到。