• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

CD44 的 v8-10 变异体同工型在正常人类结肠干细胞龛中选择性表达，并且在肿瘤发展过程中在结肠癌中经常过表达。

The v8-10 variant isoform of CD44 is selectively expressed in the normal human colonic stem cell niche and frequently is overexpressed in colon carcinomas during tumor development.

机构信息

Center for Translational Cancer Research, Helen F. Graham Cancer Center & Research Institute, Newark, DE, USA.

Department of Biologic Sciences, University of Delaware, Newark, DE, USA.

出版信息

Cancer Biol Ther. 2023 Dec 31;24(1):2195363. doi: 10.1080/15384047.2023.2195363.

DOI:10.1080/15384047.2023.2195363
PMID:37005380
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10072056/
Abstract

CD44 protein and its variant isoforms are expressed in cancer stem cells (CSCs), and various CD44 isoforms can have different functional roles in cells. Our goal was to investigate how different CD44 isoforms contribute to the emergence of stem cell (SC) overpopulation that drives colorectal cancer (CRC) development. Specific CD44 variant isoforms are selectively expressed in normal colonic SCs and become overexpressed in CRCs during tumor development. We created a unique panel of anti-CD44 rabbit genomic antibodies to 16 specific epitopes that span the entire length of the CD44 molecule. Our panel was used to comprehensively investigate the expression of different CD44 isoforms in matched pairs ( = 10) of malignant colonic tissue and adjacent normal mucosa, using two (IHC & IF) immunostaining approaches. We found that: ) CD44v8-10 is selectively expressed in the normal human colonic SC niche; ) CD44v8-10 is co-expressed with the SC markers ALDH1 and LGR5 in normal and malignant colon tissues; ) colon carcinoma tissues frequently (80%) stain for CD44v8-10 while staining for CD44v6 was less frequent (40%). Given that CD44v8-10 expression is restricted to cells in the normal human colonic SC niche and CD44v8-10 expression progressively increases during CRC development, CD44v8-10 expression likely contributes to the SC overpopulation that drives the development and growth of colon cancers. Since the CD44 variant v8-10 epitope is located on CD44's extracellular region, it offers great promise for targeted anti-CSC treatment approaches.

摘要

CD44 蛋白及其变异体同工型在癌症干细胞 (CSC) 中表达，并且各种 CD44 同工型在细胞中可能具有不同的功能作用。我们的目标是研究不同的 CD44 同工型如何促成驱动结直肠癌 (CRC) 发展的干细胞 (SC) 过度增殖的出现。特定的 CD44 变体同工型在正常结肠 SC 中特异性表达，并在肿瘤发展过程中在 CRC 中过度表达。我们创建了一组针对 CD44 分子全长的 16 个特异性表位的独特兔基因组抗 CD44 抗体。我们的小组使用两种（免疫组织化学和免疫荧光）免疫染色方法，在恶性结肠组织和相邻正常粘膜的 10 对匹配对中全面研究不同 CD44 同工型的表达。我们发现：（1）CD44v8-10 选择性地在正常人类结肠 SC 生态位中表达；（2）CD44v8-10 与 SC 标记物 ALDH1 和 LGR5 在正常和恶性结肠组织中共同表达；（3）结肠癌组织经常（80%）染色 CD44v8-10，而染色 CD44v6 则较少（40%）。鉴于 CD44v8-10 的表达仅限于正常人类结肠 SC 生态位中的细胞，并且 CD44v8-10 的表达在 CRC 发展过程中逐渐增加，CD44v8-10 的表达可能有助于驱动结肠癌发生和生长的 SC 过度增殖。由于 CD44 变体 v8-10 表位位于 CD44 的细胞外区，因此它为靶向抗 CSC 治疗方法提供了巨大的希望。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6a9/10072056/76afb8a3a712/KCBT_A_2195363_F0006_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6a9/10072056/1111e608d103/KCBT_A_2195363_F0001_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6a9/10072056/150062bda978/KCBT_A_2195363_F0002_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6a9/10072056/c76eddd1299e/KCBT_A_2195363_F0003_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6a9/10072056/c928e3d27451/KCBT_A_2195363_F0004_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6a9/10072056/a82bd08e7d36/KCBT_A_2195363_F0005_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6a9/10072056/76afb8a3a712/KCBT_A_2195363_F0006_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6a9/10072056/1111e608d103/KCBT_A_2195363_F0001_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6a9/10072056/150062bda978/KCBT_A_2195363_F0002_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6a9/10072056/c76eddd1299e/KCBT_A_2195363_F0003_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6a9/10072056/c928e3d27451/KCBT_A_2195363_F0004_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6a9/10072056/a82bd08e7d36/KCBT_A_2195363_F0005_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6a9/10072056/76afb8a3a712/KCBT_A_2195363_F0006_OC.jpg

相似文献

1
The v8-10 variant isoform of CD44 is selectively expressed in the normal human colonic stem cell niche and frequently is overexpressed in colon carcinomas during tumor development.CD44 的 v8-10 变异体同工型在正常人类结肠干细胞龛中选择性表达，并且在肿瘤发展过程中在结肠癌中经常过表达。
Cancer Biol Ther. 2023 Dec 31;24(1):2195363. doi: 10.1080/15384047.2023.2195363.
2
Δ133p53α and Δ160p53α isoforms of the tumor suppressor protein p53 exert dominant-negative effect primarily by co-aggregation.肿瘤抑制蛋白p53的Δ133p53α和Δ160p53α亚型主要通过共聚集发挥显性负效应。
Elife. 2025 Jul 21;14:RP106469. doi: 10.7554/eLife.106469.
3
Can a Liquid Biopsy Detect Circulating Tumor DNA With Low-passage Whole-genome Sequencing in Patients With a Sarcoma? A Pilot Evaluation.液体活检能否通过低深度全基因组测序检测肉瘤患者的循环肿瘤DNA？一项初步评估。
Clin Orthop Relat Res. 2025 Jan 1;483(1):39-48. doi: 10.1097/CORR.0000000000003161. Epub 2024 Jun 21.
4
The E2F4 transcriptional repressor is a key mechanistic regulator of colon cancer resistance to (CPT-11).E2F4转录抑制因子是结肠癌对伊立替康（CPT - 11）耐药性的关键机制调节因子。
bioRxiv. 2025 Jan 24:2025.01.22.633435. doi: 10.1101/2025.01.22.633435.
5
The clinicopathologic and prognostic value of CD44 expression in patients with non-small cell lung cancer: A systematic review and meta-analysis.CD44表达在非小细胞肺癌患者中的临床病理及预后价值：一项系统评价和Meta分析
Mol Cell Probes. 2025 Jun;81:102028. doi: 10.1016/j.mcp.2025.102028. Epub 2025 Mar 24.
6
EORTC guidelines for the use of erythropoietic proteins in anaemic patients with cancer: 2006 update.欧洲癌症研究与治疗组织（EORTC）癌症贫血患者促红细胞生成蛋白使用指南：2006年更新版
Eur J Cancer. 2007 Jan;43(2):258-70. doi: 10.1016/j.ejca.2006.10.014. Epub 2006 Dec 19.
7
Exploring potential therapeutic targets for colorectal tumors based on whole genome sequencing of colorectal tumors and paracancerous tissues.基于结直肠癌及癌旁组织全基因组测序探索结直肠癌潜在治疗靶点
Front Mol Biosci. 2025 Jul 4;12:1605117. doi: 10.3389/fmolb.2025.1605117. eCollection 2025.
8
The Black Book of Psychotropic Dosing and Monitoring.《精神药物剂量与监测黑皮书》
Psychopharmacol Bull. 2024 Jul 8;54(3):8-59.
9
Interventions for promoting habitual exercise in people living with and beyond cancer.促进癌症患者及康复者进行习惯性锻炼的干预措施。
Cochrane Database Syst Rev. 2018 Sep 19;9(9):CD010192. doi: 10.1002/14651858.CD010192.pub3.
10
CD44 expression is predictive of poor prognosis in pharyngolaryngeal cancer: systematic review and meta-analysis.CD44 表达与咽喉癌不良预后相关：系统评价和荟萃分析。
Tohoku J Exp Med. 2014 Jan;232(1):9-19. doi: 10.1620/tjem.232.9.

引用本文的文献

1
Combined Hyaluronic Acid Nanobioconjugates Impair CD44-Signaling for Effective Treatment Against Obesity: A Review of Comparison with Other Actors.联合透明质酸纳米生物共轭物通过损害CD44信号传导实现对肥胖症的有效治疗：与其他作用物的比较综述
Int J Nanomedicine. 2025 Aug 21;20:10101-10126. doi: 10.2147/IJN.S529250. eCollection 2025.
2
Targeting LHPP in neoadjuvant chemotherapy resistance of gastric cancer: insights from single-cell and multi-omics data on tumor immune microenvironment and stemness characteristics.靶向LHPP在胃癌新辅助化疗耐药中的作用：来自肿瘤免疫微环境和干性特征的单细胞及多组学数据的见解
Cell Death Dis. 2025 Apr 16;16(1):306. doi: 10.1038/s41419-025-07614-z.
3

本文引用的文献

1
Overexpression of CD44v8-10 in Colon Polyps-A Possible Key to Early Diagnosis.CD44v8-10 在结肠息肉中的过表达——早期诊断的一个可能关键。
Pathol Oncol Res. 2021 Mar 30;27:614281. doi: 10.3389/pore.2021.614281. eCollection 2021.
2
CD44 as a tumor biomarker and therapeutic target.CD44作为一种肿瘤生物标志物和治疗靶点。
Exp Hematol Oncol. 2020 Dec 10;9(1):36. doi: 10.1186/s40164-020-00192-0.
3
Functional analysis of CD44 variants and xCT in canine tumours.CD44 变体和 xCT 在犬肿瘤中的功能分析。
CD44 variant exons induce chemoresistance by modulating cell death pathways.
CD44可变外显子通过调节细胞死亡途径诱导化学抗性。
Front Cell Dev Biol. 2025 Mar 6;13:1508577. doi: 10.3389/fcell.2025.1508577. eCollection 2025.
4
CD44: A New Prognostic Marker in Colorectal Cancer?CD44：结直肠癌的一种新的预后标志物？
Cancers (Basel). 2024 Apr 19;16(8):1569. doi: 10.3390/cancers16081569.
5
RNA-binding proteins regulating the CD44 alternative splicing.调控CD44可变剪接的RNA结合蛋白
Front Mol Biosci. 2023 Dec 1;10:1326148. doi: 10.3389/fmolb.2023.1326148. eCollection 2023.
6
High OCT4 Expression Might Be Associated with an Aggressive Phenotype in Rectal Cancer.高OCT4表达可能与直肠癌的侵袭性表型相关。
Cancers (Basel). 2023 Jul 23;15(14):3740. doi: 10.3390/cancers15143740.
Vet Med Sci. 2021 Mar;7(2):577-585. doi: 10.1002/vms3.397. Epub 2020 Nov 18.
4
APC mutations in human colon lead to decreased neuroendocrine maturation of ALDH+ stem cells that alters GLP-2 and SST feedback signaling: Clue to a link between WNT and retinoic acid signalling in colon cancer development.APC 突变导致人结肠中的 ALDH+干细胞神经内分泌成熟减少，改变 GLP-2 和 SST 反馈信号：提示 WNT 和视黄酸信号在结肠癌发展中的联系。
PLoS One. 2020 Oct 28;15(10):e0239601. doi: 10.1371/journal.pone.0239601. eCollection 2020.
5
CD44v8-10 mRNA contained in serum exosomes as a diagnostic marker for docetaxel resistance in prostate cancer patients.血清外泌体中含有的CD44v8-10 mRNA作为前列腺癌患者多西他赛耐药的诊断标志物。
Heliyon. 2020 Jul 2;6(7):e04138. doi: 10.1016/j.heliyon.2020.e04138. eCollection 2020 Jul.
6
CD44v8-10 and CD44s Are Age-dependently Expressed in Primary Cultured Papillary Thyroid Carcinoma Cells and Are Associated with Cell Proliferation.CD44v8-10和CD44s在原代培养的甲状腺乳头状癌细胞中呈年龄依赖性表达，并与细胞增殖相关。
Kobe J Med Sci. 2019 Apr 25;65(1):E1-E9.
7
CD44 splice isoform switching determines breast cancer stem cell state.CD44 剪接异构体转换决定乳腺癌干细胞状态。
Genes Dev. 2019 Feb 1;33(3-4):166-179. doi: 10.1101/gad.319889.118. Epub 2019 Jan 28.
8
High expression level of CD44v8-10 in cancer stem-like cells is associated with poor prognosis in esophageal squamous cell carcinoma patients treated with chemoradiotherapy.癌症干细胞样细胞中CD44v8-10的高表达水平与接受放化疗的食管鳞状细胞癌患者的不良预后相关。
Oncotarget. 2018 Oct 9;9(79):34876-34888. doi: 10.18632/oncotarget.26172.
9
The biology and role of CD44 in cancer progression: therapeutic implications.CD44 在癌症进展中的生物学和作用：治疗意义。
J Hematol Oncol. 2018 May 10;11(1):64. doi: 10.1186/s13045-018-0605-5.
10
Variant isoforms of CD44 involves acquisition of chemoresistance to cisplatin and has potential as a novel indicator for identifying a cisplatin-resistant population in urothelial cancer.CD44 的变异异构体参与了对顺铂的化疗耐药性的获得，并且有可能成为鉴定膀胱癌中顺铂耐药人群的新型标志物。
BMC Cancer. 2018 Jan 31;18(1):113. doi: 10.1186/s12885-018-3988-3.