Zheng Zi-Xiu, Qiu Li-Sha, Zheng Kai, Guo Lan-Ping, Cui Xiu-Ming, Nian Hong-Juan, Li Ying-Cai, Huang Shao-Jun, Yang Ye
Faculty of Life Science and Technology, Kunming University of Science and Technology Kunming 650500, China Yunnan Provincial Key Laboratory of Panax notoginseng Kunming 650500, China.
National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
Zhongguo Zhong Yao Za Zhi. 2023 Mar;48(5):1203-1211. doi: 10.19540/j.cnki.cjcmm.20221203.101.
To study the residue and dietary risk of propiconazole in Panax notoginseng and the effects on physiological and bioche-mical properties of P. notoginseng, we conducted foliar spraying of propiconazole on P. notoginseng in pot experiments. The physiolo-gical and biochemical properties studied included leaf damage, osmoregulatory substance content, antioxidant enzyme system, non-enzymatic system, and saponin content in the main root. The results showed that at the same application concentration, the residual amount of propiconazole in each part of P. notoginseng increased with the increase in the times of application and decreased with the extension of harvest interval. After one-time application of propiconazole according to the recommended dose(132 g·hm(-2)) for P. ginseng, the half-life was 11.37-13.67 days. After 1-2 times of application in P. notoginseng, propiconazole had a low risk of dietary intake and safety threat to the population. The propiconazole treatment at the recommended concentration and above significantly increased the malondialdehyde(MDA) content, relative conductivity, and osmoregulatory substances and caused the accumulation of reactive oxygen species in P. notoginseng leaves. The propiconazole treatment at half(66 g·hm(-2)) of the recommended dose for P. ginseng significantly increased the activities of superoxide dismutase(SOD), peroxidase(POD), and catalase(CAT) in P. notoginseng leaves. The propiconazole treatment at 132 g·hm(-2) above inhibited the activities of glutathione reductase(GR) and glutathione S-transferase(GST), thereby reducing glutathione(GSH) content. Proconazole treatment changed the proportion of 5 main saponins in the main root of P. notoginseng. The treatment with 66 g·hm(-2) propiconazole promoted the accumulation of saponins, while that with 132 g·hm(-2) and above propiconazole significantly inhibited the accumulation of saponins. In summary, using propiconazole at 132 g·hm(-2) to prevent and treat P. notoginseng diseases will cause stress on P. notoginseng, while propiconazole treatment at 66 g·hm~(-2) will not cause stress on P. notoginseng but promote the accumulation of saponins. The effect of propiconazole on P. notoginseng diseases remains to be studied.
为研究丙环唑在三七中的残留量、膳食风险及其对三七生理生化特性的影响，我们通过盆栽试验对三七进行丙环唑叶面喷施。所研究的生理生化特性包括叶片损伤、渗透调节物质含量、抗氧化酶系统、非酶系统以及主根中的皂苷含量。结果表明，在相同施药浓度下，三七各部位丙环唑残留量随施药次数增加而增加，随收获间隔延长而降低。按照人参推荐剂量（132 g·hm⁻²）一次性施用丙环唑后，半衰期为11.37 - 13.67天。在三七上施用1 - 2次丙环唑后，其膳食摄入风险较低，对人群安全威胁较小。推荐浓度及以上的丙环唑处理显著增加了三七叶片中丙二醛（MDA）含量、相对电导率和渗透调节物质，并导致活性氧积累。人参推荐剂量一半（66 g·hm⁻²）的丙环唑处理显著增加了三七叶片中超氧化物歧化酶（SOD）、过氧化物酶（POD）和过氧化氢酶（CAT）的活性。132 g·hm⁻²及以上的丙环唑处理抑制了谷胱甘肽还原酶（GR）和谷胱甘肽S - 转移酶（GST）的活性，从而降低了谷胱甘肽（GSH）含量。丙环唑处理改变了三七主根中5种主要皂苷的比例。66 g·hm⁻²丙环唑处理促进了皂苷积累，而132 g·hm⁻²及以上丙环唑处理显著抑制了皂苷积累。综上所述，以132 g·hm⁻²使用丙环唑防治三七病害会对三七造成胁迫，而66 g·hm⁻²丙环唑处理不会对三七造成胁迫反而促进皂苷积累。丙环唑对三七病害的防治效果还有待研究。
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