Department of Conservative Dentistry and Endodontics, Amrita School of Dentistry, Kochi, Kerala, India.
Indian J Dent Res. 2022 Oct-Dec;33(4):440-444. doi: 10.4103/ijdr.ijdr_10_22.
This study aims to evaluate the antimicrobial efficacy of certain intracanal medicaments against Candida albicans and Enterococcus faecalis.
Freshly extracted 120 single rooted mandibular premolars were selected for the study. Teeth were decoronated and cleaning and shaping was done up to F3 universal protaper system and were divided mainly into two groups: Candida albicans (C. albicans) (n = 60) and Enterococcus faecalis (E. faecalis) (n = 60). The medicaments include: G1: chlorhexidine + calcium hydroxide, G2: sodium hypochlorite + calcium hydroxide, G3: 2% chlorhexidine gel, G4: octenisept, G5: 0.1% octenisept solution + calcium hydroxide, and G6: physiologic saline (n = 5). Teeth were contaminated with Enterococcus faecalis and Candida albicans which were cultured, respectively, in brain heart infusion and Sabouraud's dextrose agar for 21 days followed by intracanal medication and colony forming units were counted on the second and seventh day. Statistical analysis was done using Analysis of Variance (ANOVA) and Tukey's post hoc test.
Against C. albicans, CHX + CH, 2% CHX gel, 0.1% octenidine (OCT) gel and OCT + CH showed statistically significant differences on the 2 and 7 day. But against Enterococcus faecalis, only 0.1% OCT gel and 2% CHX gel showed statistically significant differences on the 2 and 7 day. Among all the groups, 0.1% OCT gel and 2% CHX gel showed predominant antimicrobial efficacy.
From the limitations of the current study, all the medicaments showed antimicrobial effect against Candida albicans and Enterococcus faecalis on the 2 and 7 day with higher microbial inhibition on the 7 day.
本研究旨在评估某些根管内药物对白色念珠菌和粪肠球菌的抗菌效果。
本研究选取了 120 颗新鲜下颌前磨牙进行研究。牙齿被截冠，使用 F3 通用扩锉系统进行根管清洁和预备，主要分为两组：白色念珠菌（C. albicans）组（n=60）和粪肠球菌（E. faecalis）组（n=60）。药物包括：G1：洗必泰+氢氧化钙，G2：次氯酸钠+氢氧化钙，G3：2%洗必泰凝胶，G4：奥替尼啶，G5：0.1%奥替尼啶溶液+氢氧化钙，G6：生理盐水（n=5）。将牙齿分别用粪肠球菌和白色念珠菌污染，在脑心浸液和沙氏葡萄糖琼脂中培养 21 天，然后进行根管内药物治疗，并在第 2 天和第 7 天计数菌落形成单位。使用方差分析（ANOVA）和 Tukey 事后检验进行统计分析。
针对白色念珠菌，CHX+CH、2%CHX 凝胶、0.1%奥替尼啶凝胶和 OCT+CH 在第 2 天和第 7 天均有统计学差异。但针对粪肠球菌，只有 0.1%奥替尼啶凝胶和 2%CHX 凝胶在第 2 天和第 7 天有统计学差异。在所有组中，0.1%奥替尼啶凝胶和 2%CHX 凝胶显示出主要的抗菌效果。
从本研究的局限性来看，所有药物在第 2 天和第 7 天都对白色念珠菌和粪肠球菌有抗菌作用，第 7 天的微生物抑制作用更高。
