不同根管内药物对的抗真菌效果：一项研究。

Antifungal Effectiveness of Various Intracanal Medicaments against : An Study.

机构信息

Department of Conservative Dentistry and Endodontics, Triveni Institute of Dental Sciences, Hospital and Research Centre, Bilaspur, Chhattisgarh, India, Phone: +91 9685514220, e-mail:

Department of Conservative Dentistry and Endodontics, Meghna Institute of Dental Sciences, Nizamabad, Telangana, India.

出版信息

J Contemp Dent Pract. 2020 Sep 1;21(9):1042-1047.

PMID:33568593

Abstract

AIM AND OBJECTIVE

To evaluate the antifungal efficiency of various intracanal medicaments against .

MATERIALS AND METHODS

One-hundred and forty extracted human mandibular premolar teeth were decoronated, and the biomechanical preparation was done in crown-down technique. 10 μL culture suspension of was placed into the prepared root canal space of all the teeth. After 21 days of incubation, all the teeth were randomly divided into 7 groups with 20 teeth per each group. Group I: triple antibiotic powder (TAP) mixed with 3% chitosan solution; group II: TAP mixed with macrogol-propylene (MP) glycol; group III: chlorhexidine-guttapercha (CHX-GP); group IV: Vitapex; group V: 2% chlorhexidine gel; group VI: calcium hydroxide paste; group VII: normal saline with cotton (positive control) were used as intracanal medicaments, and the samples were incubated for 14 days. Intracanal medicaments were then completely removed using the canal brush. Dentinal chips were harvested from the walls of the root canal space in all samples using Gates-Glidden drills, were transferred into test tubes containing saline, and were serially diluted and placed in 140 Sabouraud dextrose agar plates, incubated at 37°C for 48 hours. Colony forming units (CFUs) of were then counted using the digital colony counter.

RESULTS

One-way ANOVA test showed statistically significant difference among the seven groups, as the value was < 0.001. Tukey's test showed intergroup comparison between group I and group V; group II and group III were statistically nonsignificant as value was >0.05.

CONCLUSION

2% chlorhexidine gel and TAP mixed with 3% chitosan solution showed superior antifungal efficiency against .

CLINICAL SIGNIFICANCE

Chitosan solution's inherent antifungal efficiency and slow and controlled drug release make it as an effective alternate carrier in mixing it with TAP instead of mixing TAP with MP.

摘要

目的

评估各种根管内药物对的抗真菌效果。

材料和方法

将 140 颗人类下颌前磨牙牙冠截断，采用冠向下技术进行生物力学预备。将 10 μL 培养悬液的放置到所有牙齿的预备根管空间中。经过 21 天的孵育，所有牙齿被随机分为 7 组，每组 20 颗牙齿。第 I 组：三抗生素粉（TAP）与 3%壳聚糖溶液混合；第 II 组：TAP 与聚丙二醇（MP）混合；第 III 组：洗必泰-牙胶（CHX-GP）；第 IV 组：Vitapex；第 V 组：2%洗必泰凝胶；第 VI 组：氢氧化钙糊剂；第 VII 组：生理盐水加棉（阳性对照）作为根管内药物，孵育 14 天。然后使用根管刷完全清除根管内药物。使用 Gates-Glidden 钻从所有样本根管壁上采集牙本质屑，转移到含有生理盐水的试管中，连续稀释后放置在 140 个 Sabouraud 葡萄糖琼脂平板上，在 37°C 孵育 48 小时。然后使用数字菌落计数器计数的菌落形成单位（CFU）。