Xu Miao, Ji Xiaohuan, Huo Jingjing, Chen Jingjie, Liu Nian, Li Ziyue, Jia Qingyan, Sun Bin, Zhu Meifang, Li Peng
Frontiers Science Center for Flexible Electronics (FSCFE), Xi'an Institute of Flexible Electronics (IFE) & Xi'an Institute of Biomedical Materials and Engineering (IBME), Northwestern Polytechnical University (NPU), Xi'an 710072, China.
Key Laboratory of Flexible Electronics (KLOFE) and Institute of Advanced Materials (IAM), Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing Tech University, Nanjing 211816, China.
ACS Appl Mater Interfaces. 2023 Apr 12;15(14):17742-17756. doi: 10.1021/acsami.3c03247. Epub 2023 Apr 2.
Reactive oxygen species (ROS) produced by noble metallic nanoparticles under visible light is an effective way to combat drug-resistant bacteria colonized on the wound. However, the photocatalytic efficiency of noble metallic nanoparticles is limited by its self-aggregation in water media. Moreover, the fast release of noble metallic ions from nanoparticles might engender cellular toxicity and hazardous environmental issues. Herein, we chose AgNPs, the most common plasmonic noble metallic nanoparticles, as an example, modifying the surface of AgNPs with oleic acid and -butylamine and imbedded them into calcium alginate (CA) hydrogel that holds tissue adhesion, rapid hemostatic, sunlight-sensitive antibacterial and anti-inflammatory abilities, and thus effectively promotes the healing of wounds. Unlike conventional AgNP-based materials, the constrain of colloids and hydrogel networks hinders the leach of Ag. Nonetheless, the CA/Ag hydrogels exhibit on-demand photodynamic antibacterial efficacy due to the generation of ROS under visible light. In addition, the CA/Ag hydrogel can effectively stop the hemorrhage in a mouse liver bleeding model due to their skin-adaptive flexibility and tissue adhesiveness. The potent sunlight-responsive antibacterial activity of the CA/Ag hydrogel can effectively kill multidrug-resistant bacteria both (>99.999%) and (>99.9%), while the diminished Ag release guarantees its biocompatibility. The CA/Ag hydrogel significantly promotes the wound healing process by the downregulation of proinflammatory cytokines (TNF-α and IL-6) in a rodent full-thickness cutaneous wound model. Overall, the proposed multifunctional CA/Ag nanocomposite hydrogel has excellent prospects as an advanced wound dressing.
贵金属纳米颗粒在可见光下产生的活性氧（ROS）是对抗伤口上定植的耐药细菌的有效方法。然而，贵金属纳米颗粒的光催化效率受到其在水介质中自聚集的限制。此外，纳米颗粒中贵金属离子的快速释放可能会产生细胞毒性和有害的环境问题。在此，我们以最常见的等离子体贵金属纳米颗粒AgNPs为例，用油酸和丁胺修饰AgNPs的表面，并将它们嵌入具有组织粘附、快速止血、对阳光敏感的抗菌和抗炎能力的海藻酸钙（CA）水凝胶中，从而有效地促进伤口愈合。与传统的基于AgNP的材料不同，胶体和水凝胶网络的限制阻碍了Ag的浸出。尽管如此，由于在可见光下产生ROS，CA/Ag水凝胶表现出按需光动力抗菌功效。此外，CA/Ag水凝胶由于其皮肤适应性柔韧性和组织粘附性，可以在小鼠肝出血模型中有效地止血。CA/Ag水凝胶强大的阳光响应抗菌活性可以有效杀死耐多药细菌（>99.999%）和（>99.9%），同时减少的Ag释放保证了其生物相容性。在啮齿动物全层皮肤伤口模型中，CA/Ag水凝胶通过下调促炎细胞因子（TNF-α和IL-6）显著促进伤口愈合过程。总体而言，所提出的多功能CA/Ag纳米复合水凝胶作为一种先进的伤口敷料具有极好的前景。
