Parasitic nematode secreted phospholipase A suppresses cellular and humoral immunity by targeting hemocytes in .

A key aspect of parasitic nematode infection is the nematodes' ability to evade and/or suppress host immunity. This immunomodulatory ability is likely driven by the release of hundreds of excretory/secretory proteins (ESPs) during infection. While ESPs have been shown to display immunosuppressive effects on various hosts, our understanding of the molecular interactions between individual proteins released and host immunity requires further study. We have recently identified a secreted phospholipase A2 (sPLA) released from the entomopathogenic nematode (EPN) we have named Sc-sPLA. We report that Sc-sPLA increased mortality of infected with and promoted increased bacterial growth. Furthermore, our data showed that Sc-sPLA was able to downregulate both Toll and Imd pathway-associated antimicrobial peptides (AMPs) including drosomycin and defensin, in addition to suppressing phagocytosis in the hemolymph. Sc-sPLA was also found to be toxic to with the severity being both dose- and time-dependent. Collectively, our data highlighted that Sc-sPLA possessed both toxic and immunosuppressive capabilities.