Zhang Ming-Sha, Liang Si-Zhou, Zhang Wei-Guo, Chang Ya-Jun, Lei Zhongfang, Li Wen, Zhang Guo-Liang, Gao Yan
School of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, China.
Jiangsu Key Laboratory for the Research and Utilization of Plant Resources, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences (Nanjing Botanical Garden Memorial Sun Yat-Sen), Nanjing, China.
Front Microbiol. 2023 Mar 16;14:1135278. doi: 10.3389/fmicb.2023.1135278. eCollection 2023.
Farmlands fertilized with livestock manure-derived amendments have become a hot topic in the dissemination of antibiotic resistance genes (ARGs). Field ponding water connects rice paddies with surrounding water bodies, such as reservoirs, rivers, and lakes. However, there is a knowledge gap in understanding whether and how manure-borne ARGs can be transferred from paddy soil into field ponding water. Our studies suggest that the manure-derived ARGs , , , , , , and can easily be transferred into field ponding water from paddy soil. The bacterial phyla Crenarchaeota, Verrucomicrobia, Cyanobacteria, Choloroflexi, Acidobacteria, Firmicutes, Bacteroidetes, and Actinobacteria are potential hosts of ARGs. Opportunistic pathogens detected in both paddy soil and field ponding water showed robust correlations with ARGs. Network co-occurrence analysis showed that mobile genetic elements (MGEs) were strongly correlated with ARGs. Our findings highlight that manure-borne ARGs and antibiotic-resistant bacteria in paddy fields can conveniently disseminate to the surrounding waterbodies through field ponding water, posing a threat to public health. This study provides a new perspective for comprehensively assessing the risk posed by ARGs in paddy ecosystems.
施用源自畜禽粪便的改良剂的农田已成为抗生素抗性基因（ARGs）传播方面的一个热门话题。田面水将稻田与周围水体（如水库、河流和湖泊）连接起来。然而，在理解粪便携带的ARGs是否以及如何从稻田土壤转移到田面水中方面存在知识空白。我们的研究表明，源自粪便的ARGs 、 、 、 、 、 、 和 可以很容易地从稻田土壤转移到田面水中。泉古菌门、疣微菌门、蓝细菌门、绿弯菌门、酸杆菌门、厚壁菌门、拟杆菌门和放线菌门是ARGs的潜在宿主。在稻田土壤和田面水中检测到的机会性病原体与ARGs表现出很强的相关性。网络共现分析表明，移动遗传元件（MGEs）与ARGs高度相关。我们的研究结果突出表明，稻田中粪便携带的ARGs和抗生素抗性细菌可通过田面水便利地传播到周围水体，对公众健康构成威胁。本研究为全面评估稻田生态系统中ARGs所带来的风险提供了一个新视角。
