Du Ting-Ting, Liu Xiao-Chen, He Yu, Gao Xiong, Liu Zhen-Zhen, Wang Zheng-Li, Li Lu-Quan
Neonatal Diagnosis and Treatment Centre of Children's Hospital of Chongqing Medical University, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, International Science and Technology Cooperation Base of Child Development and Critical Disorders, Chongqing Key Laboratory of Pediatrics, Chongqing, China.
Jiangxi Hospital Affiliated Children's Hospital of Chongqing Medical University, Nanchang, China.
Front Microbiol. 2023 Mar 15;14:1119981. doi: 10.3389/fmicb.2023.1119981. eCollection 2023.
We aimed to explore the value of gut microbiota and tricarboxylic acid (TCA) metabolites in early diagnosis of necrotizing enterocolitis (NEC) among infants with abdominal manifestations.
Thirty-two preterm infants with abdominal manifestations at gestational age ≤ 34 weeks were included in the study and were divided into non-NEC ( = 16) and NEC ( = 16) groups. Faecal samples were collected when the infants were enrolled. The gut microbiota was analysed with high-throughput sequencing, and TCA metabolites were measured with multiple reaction monitoring (MRM) targeted metabolomics. Receiver operating characteristic (ROC) curves were generated to explore the predictive value of the obtained data.
There was no significant difference in alpha diversity or beta diversity between the two groups ( > 0.05). At the phylum level, increased, and decreased in the NEC group ( < 0.05). At the genus level, and decreased significantly, and at the species level, , and decreased in the NEC group ( < 0.05). Further Linear discriminant analysis effect sizes () analysis showed that the change in at the phylum level and and at the genus level scored higher than 4. The concentrations of succinate, L-malic acid and oxaloacetate in the NEC group significantly increased ( < 0.05), and the areas under the ROC curve for these metabolites were 0.6641, 0.7617, and 0.7344, respectively.
Decreased , and at the species level as well as the increase in the contents of some TCA metabolites, including succinate, L-malic acid and oxaloacetate, have potential value for the early diagnosis of NEC.
我们旨在探讨肠道微生物群和三羧酸（TCA）代谢物在有腹部表现的婴儿坏死性小肠结肠炎（NEC）早期诊断中的价值。
本研究纳入了32例胎龄≤34周且有腹部表现的早产儿，分为非NEC组（n = 16）和NEC组（n = 16）。婴儿入组时采集粪便样本。采用高通量测序分析肠道微生物群，采用多反应监测（MRM）靶向代谢组学测量TCA代谢物。绘制受试者工作特征（ROC）曲线以探索所得数据的预测价值。
两组之间的α多样性或β多样性无显著差异（P>0.05）。在门水平上，NEC组中[具体菌门名称1]增加，[具体菌门名称2]减少（P<0.05）。在属水平上，[具体属名称1]和[具体属名称2]显著减少，在种水平上，NEC组中[具体种名称1]、[具体种名称2]和[具体种名称3]减少（P<0.05）。进一步的线性判别分析效应大小（LEfSe）分析表明，门水平上[具体菌门名称1]的变化以及属水平上[具体属名称1]和[具体属名称2]的得分高于4。NEC组中琥珀酸、L-苹果酸和草酰乙酸的浓度显著升高（P<0.05），这些代谢物的ROC曲线下面积分别为0.6641、0.7617和0.7344。
种水平上[具体种名称1]、[具体种名称2]和[具体种名称3]的减少以及一些TCA代谢物含量的增加，包括琥珀酸、L-苹果酸和草酰乙酸，对NEC的早期诊断具有潜在价值。
