Liu Xiao-Chen, Du Ting-Ting, Gao Xiong, Zhao Wen-Jing, Wang Zheng-Li, He Yu, Bao Lei, Li Lu-Quan
Neonatal Diagnosis and Treatment Center of Children's Hospital of Chongqing Medical University, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, International Science and Technology Cooperation Base of Child Development and Critical Disorders, Chongqing Key Laboratory of Pediatrics, Chongqing, China.
Front Microbiol. 2022 Aug 17;13:969656. doi: 10.3389/fmicb.2022.969656. eCollection 2022.
Dysbacteriosis is thought to play an important role in the pathogenesis of necrotizing enterocolitis (NEC). We aimed to identify new biomarkers among gut microbiota and short-chain fatty acids (SCFAs) for the early prediction of NEC.
Thirty-four preterm infants with gestational ages of ≤ 34 weeks who developed gastrointestinal symptoms were divided into the NEC group ( = 17) and non-NEC group ( = 17). In the NEC group, the gut microbiota and SCFAs in feces were assessed when the infants were enrolled (Group P) and when they were diagnosed with NEC (Group N). In the non-NEC group, samples were assessed when the infants were enrolled (Group C).
The Ace and Chao1 indices were higher in Group P than in Group C ( < 0.05), and there was no difference between Groups C and N or between Groups P and N ( > 0.05). There was no significant difference in the Simpson and Shannon indices among Groups C, P and N ( > 0.05). The four main phyla showed no differences ( > 0.05) in composition, while at the genus level, compared with Group C, in Group P, , and were increased, while and were decreased ( < 0.05). At the species level, and increased, while decreased ( < 0.05). In Group N, at the genus level, , and increased ( < 0.05). Compared with those in Group C, the levels of acetic acid, propanoic acid and butyric acid decreased significantly in Groups P and N ( < 0.05), and the areas under the curves (AUCs) of these three SCFAs between groups C and P were 0.73, 0.70, and 0.68, respectively.
The increase in and and decrease in , as well as the decrease in acetic, propionic and butyric acids, may help in the early prediction of NEC.
肠道菌群失调被认为在坏死性小肠结肠炎（NEC）的发病机制中起重要作用。我们旨在确定肠道微生物群和短链脂肪酸（SCFAs）中的新生物标志物，用于NEC的早期预测。
34例胎龄≤34周出现胃肠道症状的早产儿被分为NEC组（n = 17）和非NEC组（n = 17）。在NEC组中，婴儿入组时（P组）和被诊断为NEC时（N组）评估粪便中的肠道微生物群和SCFAs。在非NEC组中，婴儿入组时（C组）评估样本。
P组的Ace和Chao1指数高于C组（P < 0.05），C组和N组之间以及P组和N组之间无差异（P > 0.05）。C组、P组和N组之间的Simpson和Shannon指数无显著差异（P > 0.05）。四个主要门在组成上无差异（P > 0.05），而在属水平上，与C组相比，P组中，[具体菌属1]、[具体菌属2]和[具体菌属3]增加，而[具体菌属4]和[具体菌属5]减少（P < 0.05）。在种水平上，[具体菌种1]和[具体菌种2]增加，而[具体菌种3]减少（P < 0.05）。在N组中，在属水平上，[具体菌属6]、[具体菌属7]和[具体菌属8]增加（P < 0.05）。与C组相比，P组和N组中乙酸、丙酸和丁酸水平显著降低（P < 0.05），这三种SCFAs在C组和P组之间的曲线下面积（AUCs）分别为0.73、0.70和0.68。
[具体菌属1]和[具体菌属2]增加以及[具体菌属3]减少，以及乙酸、丙酸和丁酸减少，可能有助于NEC的早期预测。
