Niedźwiedzka-Rystwej Paulina, Deptuła Wiesław
Institute of Biology, University of Szczecin, 71-412 Szczecin, Poland.
Institute of Veterinary Sciences, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University in Toruń, 87-100 Toruń, Poland.
J Vet Res. 2023 Feb 28;67(1):41-47. doi: 10.2478/jvetres-2023-0008. eCollection 2023 Mar.
is a single-stranded RNA virus causing an acute fatal disease in wild and domestic rabbits around the world. Studies have shown that the pivotal process impacting the immune response against the disease is apoptosis, registered mainly in hepatocytes and in peripheral blood, together with an increased number of cytotoxic lymphocytes (CTLs). It is known that cytotoxic lymphocytes can induce target cells to undergo apoptosis on the pseudoreceptor pathway, such apoptosis having been found in several acute and chronic viral infections. The study aimed to assess the crosstalk between the apoptosis of peripheral blood lymphocytes and CD8+ T lymphocytes (as CTLs) in rabbits infected with 6 GI.1a viruses.
Sixty rabbits of Polish hybrid breed comprising both sexes and weighing 3.2-4.2 kg were the experimental group, and an identical group was the control. Each of the six GI.1a viruses was inoculated into ten experimental rabbits. Control rabbits received glycerol as a placebo. Flow cytometric analysis was performed on blood from the study and control group animals for peripheral blood lymphocyte apoptosis and CTL percentage determination.
The activation of apoptosis in peripheral blood lymphocytes was recorded from 4 h post inoculation (p.i.) up to 36 h p.i. The percentage of CTLs in the total blood pool decreased from 8 to 36 h p.i. A negative correlation between apoptosis of lymphocytes and the number of CTLs was proven.
This may be the first evidence of virus-induced CTL apoptosis in GI.1a infection.
是一种单链RNA病毒，在全球野生和家养兔中引发急性致命疾病。研究表明，影响针对该疾病免疫反应的关键过程是细胞凋亡，主要在肝细胞和外周血中出现，同时细胞毒性淋巴细胞（CTLs）数量增加。已知细胞毒性淋巴细胞可通过假受体途径诱导靶细胞发生凋亡，这种凋亡在多种急性和慢性病毒感染中均有发现。本研究旨在评估感染6种GI.1a病毒的兔外周血淋巴细胞凋亡与CD8 + T淋巴细胞（作为CTLs）之间的相互作用。
60只波兰杂交品种兔，雌雄皆有，体重3.2 - 4.2千克，作为实验组，另一组相同的兔子作为对照组。将六种GI.1a病毒中的每一种接种到十只实验兔体内。对照兔接受甘油作为安慰剂。对实验组和对照组动物的血液进行流式细胞术分析，以测定外周血淋巴细胞凋亡和CTL百分比。
接种后4小时（p.i.）至36小时p.i.记录到外周血淋巴细胞凋亡激活。接种后8小时至36小时p.i.，全血池中CTL百分比下降。淋巴细胞凋亡与CTL数量之间存在负相关。
这可能是GI.1a感染中病毒诱导CTL凋亡的首个证据。
