Ataseven Veysel S, Ambarcıoğlu Pınar, Doğan Fırat
Department of Virology, Faculty of Veterinary Medicine, Hatay Mustafa Kemal University, 31040 Antakya, Hatay, Türkiye.
Department of Biostatistics, Faculty of Veterinary Medicine, Hatay Mustafa Kemal University, 31040 Antakya, Hatay, Türkiye.
J Vet Res. 2023 Mar 16;67(1):33-40. doi: 10.2478/jvetres-2023-0010. eCollection 2023 Mar.
Bovine viral diarrhoea virus (BVDV) and bovine herpesvirus (BoHV)-1 and -4 are important causes of respiratory diseases and reproductive disorders of dairy cattle worldwide.
Investigation of BVDV and BoHV-1 and -4 antibody levels in the serum and milk of dairy cattle in a group with clinical mastitis and a healthy group was undertaken using an indirect ELISA, and identification of the BoHV-4 genotypes in clinical mastitis cases was attempted by PCR and sequencing.
Antibodies specific to BVDV, BoHV-1 and BoHV-4 were detected in the serum and milk of all dairy cattle with clinical mastitis. The cut-off values for BVDV and BoHV-1 in the sera and milk were extremely high in both healthy and mastitic animals. However, BoHV-4 antibodies were detected only in the clinically mastitic cattle, and BoHV-4 levels were higher in milk than in sera among these animals. Genotypes I and II of BoHV-4 were detected in the milk samples of four seropositive cows with clinical mastitis from the same herd.
The results of this investigation demonstrate that clinical mastitis cases in the same herd may have aetiology in different BoHV-4 genotypes.
牛病毒性腹泻病毒（BVDV）以及牛疱疹病毒（BoHV）-1和-4是全球奶牛呼吸道疾病和繁殖障碍的重要病因。
采用间接ELISA法对一组临床型乳腺炎奶牛和健康奶牛的血清及乳汁中的BVDV以及BoHV-1和-4抗体水平进行调查，并尝试通过PCR和测序对临床型乳腺炎病例中的BoHV-4基因型进行鉴定。
在所有临床型乳腺炎奶牛的血清和乳汁中均检测到了针对BVDV、BoHV-1和BoHV-4的抗体。在健康奶牛和患乳腺炎奶牛中，血清和乳汁中BVDV和BoHV-1的临界值都极高。然而，仅在临床型乳腺炎奶牛中检测到了BoHV-4抗体，且在这些奶牛中，乳汁中的BoHV-4水平高于血清。在来自同一牛群的4头血清阳性且患有临床型乳腺炎的奶牛的乳汁样本中检测到了BoHV-4的I型和II型。
本次调查结果表明，同一牛群中的临床型乳腺炎病例可能由不同的BoHV-4基因型引起。
