Yoon Jae-Hyun, Kim Ji-Yeon, Yoo Jin-Hee, Lee Sun-Young
Department of Food and Nutrition, Chung-Ang University, 4726, Seodong-Daero, Anseong-Si, Gyeonggi-Do Republic of Korea.
Food Sci Biotechnol. 2022 Nov 29;32(5):713-721. doi: 10.1007/s10068-022-01202-z. eCollection 2023 Apr.
This study was conducted to develop a selective medium for enumerating LAB and Bifidobacteria in food samples. Thirteen media were evaluated to determine their suitability for selectively enumerating LAB (17 bacilli and 7 cocci) and Bifidobacteria (12 strains) under aerobic and anaerobic conditions. When BL, BCP, and mMRS were supplemented with propionic acid (5 ml/l) and adjusted to pH 5, the growth of all indicator microorganisms was inhibited; however, these media also inhibited the growth of certain LAB and Bifidobacterial strains. Using propionic acid, the pH levels of BL, BCP, and mMRS were adjusted to pH 5.2, 6.0, and 6.0, respectively. These media inhibited the growth of all indicator microorganisms, whereas they did not inhibit any of the LAB and Bifidobacteria strains under anaerobic conditions. Overall, BLP (pH 5.8) lacking blood showed significantly higher bacterial counts compared with other media in food products. Further analyses indicated that BLP (pH 5.8) was the most suitable medium for enumerating LAB and Bifidobacteria in food.
The online version contains supplementary material available at 10.1007/s10068-022-01202-z.
本研究旨在开发一种用于计数食品样本中乳酸菌（LAB）和双歧杆菌的选择性培养基。对13种培养基进行了评估，以确定它们在需氧和厌氧条件下选择性计数LAB（17株杆菌和7株球菌）和双歧杆菌（12株）的适用性。当在BL、BCP和mMRS中添加丙酸（5 ml/l）并将pH值调至5时，所有指示微生物的生长均受到抑制；然而，这些培养基也抑制了某些LAB和双歧杆菌菌株的生长。使用丙酸将BL、BCP和mMRS的pH值分别调至5.2、6.0和6.0。这些培养基抑制了所有指示微生物的生长，而在厌氧条件下它们并未抑制任何LAB和双歧杆菌菌株的生长。总体而言，不含血液的BLP（pH 5.8）在食品中的细菌计数显著高于其他培养基。进一步分析表明，BLP（pH 5.8）是计数食品中LAB和双歧杆菌的最合适培养基。
在线版本包含可在10.1007/s10068-022-01202-z获取的补充材料。
