Bae Jin-Woo, Rhee Sung-Keun, Park Ja Ryeong, Chung Won-Hyong, Nam Young-Do, Lee Insun, Kim Hongik, Park Yong-Ha
Biological Resources Center, Korea Research Institute of Bioscience and Biotechnology, Eundong 52, Yusong, Daejeon, South Korea.
Appl Environ Microbiol. 2005 Dec;71(12):8825-35. doi: 10.1128/AEM.71.12.8825-8835.2005.
The genome-probing microarray (GPM) was developed for quantitative, high-throughput monitoring of community dynamics in lactic acid bacteria (LAB) fermentation through the deposit of 149 microbial genomes as probes on a glass slide. Compared to oligonucleotide microarrays, the specificity of GPM was remarkably increased to a species-specific level. GPM possesses about 10- to 100-fold higher sensitivity (2.5 ng of genomic DNA) than the currently used 50-mer oligonucleotide microarrays. Since signal variation between the different genomes was very low compared to that of cDNA or oligonucleotide-based microarrays, the capacity of global quantification of microbial genomes could also be observed in GPM hybridization. In order to assess the applicability of GPMs, LAB community dynamics were monitored during the fermentation of kimchi, a traditional Korean food. In this work, approximately 100 diverse LAB species could be quantitatively analyzed as actively involved in kimchi fermentation.
基因组探测微阵列（GPM）是通过将149个微生物基因组作为探针沉积在载玻片上，用于定量、高通量监测乳酸菌（LAB）发酵过程中群落动态的技术。与寡核苷酸微阵列相比，GPM的特异性显著提高到物种特异性水平。GPM的灵敏度（2.5 ng基因组DNA）比目前使用的50聚体寡核苷酸微阵列高约10至100倍。由于与基于cDNA或寡核苷酸的微阵列相比，不同基因组之间的信号变化非常低，因此在GPM杂交中也可以观察到微生物基因组的全局定量能力。为了评估GPM的适用性，在传统韩国食品泡菜的发酵过程中监测了LAB群落动态。在这项工作中，可以定量分析约100种不同的LAB物种积极参与泡菜发酵。
