Zhao Dan, Niu Xinghua, Shang Kaijie, Sun Guozhen, Liu Shumin, Wang Zengli, Chen Anyu, Zhu Xiaoping, Zang Lianyi
College of Plant Protection, Shandong Agricultural University, Tai'an, China.
Qingdao Agricultural Technology Extension Center, Qing'dao, China.
Mol Plant Pathol. 2025 Jul;26(7):e70115. doi: 10.1111/mpp.70115.
Tomato chlorosis virus (ToCV), a phloem-restricted RNA virus within the genus Crinivirus of the family Closteroviridae, exhibits a broad host range and severely impacts the yield and quality of multiple crops. Viral infection directly alters endogenous phytohormone levels, which are intricately associated with viral mobility, replication, symptom development and defence mechanisms. Previous studies have demonstrated that GATA transcription factors regulate several hormone signalling pathways in plants. In this study, we explored the interaction between ToCV p27 and SlGATA8/NbGATA11. Results indicated that ToCV p27 interacts with an 18-amino-acid at the C-terminus of SlGATA8 and NbGATA11 proteins. Silencing and overexpressing of SlGATA8 revealed its positive role in regulating tomato defence against ToCV infection. Additionally, the interaction redirected SlGATA8's subcellular localisation to plasmodesmata. Furthermore, SlGATA8 promoted the transcriptional expression of SlSnRK2 to regulate the abscisic acid (ABA) signalling pathway. In conclusion, this study confirmed that ToCV p27 impaired the transcriptional activation activity of SlGATA8 through direct interaction, thereby inhibiting the ABA pathway and ultimately facilitating viral infection. This study established a link among virus, GATA family transcription factors and phytohormones, elucidating the molecular mechanism by which ToCV-encoded p27 protein interacts with SlGATA8 to disrupt ABA balance and promote virus infection.
番茄褪绿病毒（ToCV）是长线形病毒科毛形病毒属内一种局限于韧皮部的RNA病毒，具有广泛的寄主范围，严重影响多种作物的产量和品质。病毒感染直接改变内源植物激素水平，而植物激素水平与病毒移动、复制、症状发展及防御机制密切相关。先前的研究表明，GATA转录因子调控植物中的几种激素信号通路。在本研究中，我们探究了ToCV p27与SlGATA8/NbGATA11之间的相互作用。结果表明，ToCV p27与SlGATA8和NbGATA11蛋白C末端的一个18氨基酸序列相互作用。SlGATA8的沉默和过表达揭示了其在调控番茄抵御ToCV感染方面的积极作用。此外，这种相互作用将SlGATA8的亚细胞定位重定向至胞间连丝。此外，SlGATA8促进SlSnRK2的转录表达以调控脱落酸（ABA）信号通路。总之，本研究证实ToCV p27通过直接相互作用损害SlGATA8的转录激活活性，从而抑制ABA通路并最终促进病毒感染。本研究建立了病毒、GATA家族转录因子和植物激素之间的联系，阐明了ToCV编码的p27蛋白与SlGATA8相互作用以破坏ABA平衡并促进病毒感染的分子机制。
