Kaji Hideko, Kaji Akira
Thomas Jefferson University, Philadelphia, PA, USA.
University of Pennsylvania, Philadelphia, PA, USA.
Methods Mol Biol. 2023;2620:41-50. doi: 10.1007/978-1-0716-2942-0_6.
Following our early discovery of arginylation in 1963, we have performed several studies to correlate its activity with essential biological processes. We employed cell- and tissue-based assays to detect both the level of acceptor proteins and the level of ATE1 activity under different conditions. Remarkably, in these assays, we found a close correlation between arginylation and aging, a discovery that we believe has longer-term implications in uncovering the importance of ATE1 in normal biology and disease therapies. Here, we describe the original methods we used to measure ATE1 activity in tissues and correlate it with key biological events.
自1963年我们早期发现精氨酰化作用以来，我们开展了多项研究，以将其活性与重要的生物学过程相关联。我们采用基于细胞和组织的检测方法，来检测不同条件下受体蛋白的水平和ATE1活性水平。值得注意的是，在这些检测中，我们发现精氨酰化与衰老之间存在密切关联，我们认为这一发现对于揭示ATE1在正常生物学和疾病治疗中的重要性具有更长远的意义。在此，我们描述了我们最初用于测量组织中ATE1活性并将其与关键生物学事件相关联的方法。
