用于精氨酰化测定的重组精氨酰转移酶（ATE1）和精氨酰-tRNA合成酶（RRS）的细菌表达与纯化
Bacterial Expression and Purification of Recombinant Arginyltransferase (ATE1) and Arg-tRNA Synthetase (RRS) for Arginylation Assays.
作者信息
Wang Junling, Kashina Anna S
机构信息
University of Pennsylvania, Philadelphia, PA, USA.
Department of Animal Biology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA, USA.
出版信息
Methods Mol Biol. 2023;2620:87-91. doi: 10.1007/978-1-0716-2942-0_11.
Here, we describe the procedure for the expression and purification of recombinant ATE1 from E. coli. This method is easy and convenient and can result in one-step isolation of milligram amounts of soluble enzymatically active ATE1 at nearly 99% purity. We also describe a procedure for the expression and purification of E. coli Arg-tRNA synthetase essential for the arginylation assays described in the next two chapters.
在此，我们描述了从大肠杆菌中表达和纯化重组ATE1的方法。该方法简便易行，能够一步分离出毫克量的可溶性且具有酶活性的ATE1，纯度近99%。我们还描述了大肠杆菌精氨酸-tRNA合成酶的表达和纯化方法，该酶对于接下来两章中所述的精氨酸化测定至关重要。
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