Bechtel P J
J Biol Chem. 1979 Mar 25;254(6):1755-8.
A large polypeptide having a molecular weight of 240,000 as determined by electrophoresis in the presence of sodium dodecyl sulfate has been identified in whole cell homogenates from chick skeletal muscle myoblasts and the rat myoblast L6 cell line. A similar polypeptide was identified in both thigh and breast chicken skeletal muscle, but the latter contained less of this protein per g of tissue. Antibodies made to gizzard filamin (an actin-binding protein having a molecular weight of 240,000) cross-reacted with the partially purified Mr = 240,000 protein from chicken skeletal muscle. With use of the indirect immunofluorescence technique, the filamin antibody localized in the Z-line region of chicken skeletal muscle myofibrils. These results indicate that skeletal muscle contains a filamin-like protein that may form an integral part of the myofibril structure.
通过在十二烷基硫酸钠存在下进行电泳测定，在鸡骨骼肌成肌细胞和大鼠成肌细胞L6细胞系的全细胞匀浆中鉴定出一种分子量为240,000的大分子量多肽。在鸡大腿和胸肌骨骼肌中也鉴定出了一种类似的多肽，但后者每克组织中这种蛋白质的含量较少。针对砂囊细丝蛋白（一种分子量为240,000的肌动蛋白结合蛋白）制备的抗体与从鸡骨骼肌中部分纯化的分子量为240,000的蛋白质发生交叉反应。使用间接免疫荧光技术，细丝蛋白抗体定位于鸡骨骼肌肌原纤维的Z线区域。这些结果表明，骨骼肌含有一种细丝蛋白样蛋白，它可能是肌原纤维结构的一个组成部分。
