Berg B
J Clin Chem Clin Biochem. 1986 Jan;24(1):89-96. doi: 10.1515/cclm.1986.24.1.89.
Currently used test strip methods for the detection of glucose in urine are influenced by ascorbate and may thus give false negative results, e.g. in screening for diabetes. Six different test strips for urine glucose were evaluated for interference by ascorbate in vitro. Interference by ascorbate varied markedly, being highest at low glucose concentrations. Interference coefficients for the individual tests were calculated to serve as an approximate index of interference by ascorbate. A new test (BM 33.071, Boehringer Mannheim GmbH, currently used in Combur-9-Test/Chemstrip-9 and other multiple test strips of Boehringer Mannheim) was clearly much less influenced as no urine containing 5.5 mmol/l glucose was read as negative even at very high ascorbate concentration. Readability of test strips differed due to patchy colour reactions. Precision was good within-test strip and within-urine but markedly less between urines.
目前用于检测尿糖的试纸法受抗坏血酸盐影响，因此可能产生假阴性结果，例如在糖尿病筛查中。对六种不同的尿糖试纸进行了体外抗坏血酸盐干扰评估。抗坏血酸盐的干扰差异显著，在低葡萄糖浓度时最高。计算了各个测试的干扰系数，作为抗坏血酸盐干扰的近似指标。一种新测试（BM 33.071，勃林格殷格翰公司，目前用于Combur-9-Test/Chemstrip-9以及勃林格殷格翰的其他多项试纸）受影响明显小得多，因为即使在非常高的抗坏血酸盐浓度下，含5.5 mmol/l葡萄糖的尿液也不会被读为阴性。由于显色反应不均匀，试纸的可读性存在差异。试纸条内和尿液内的精密度良好，但不同尿液间的精密度明显较低。
