Centro de Biología Molecular "Severo Ochoa", CSIC and Universidad Autónoma de Madrid, Madrid, 28049, Spain.
Centro de Biología Molecular "Severo Ochoa", CSIC and Universidad Autónoma de Madrid, Madrid, 28049, Spain.
Dev Biol. 2023 Jun;498:61-76. doi: 10.1016/j.ydbio.2023.03.010. Epub 2023 Apr 2.
Cell division and cytoskeleton organization are fundamental processes participating in the development of Drosophila imaginal discs. In this manuscript we describe the phenotypes in the adult fly wing generated by knockdowns of 85% of Drosophila genes encoding proteins likely related to the regulation of cell division and cytoskeleton organization. We also compile a molecular classification of these proteins into classes that describe their expected or known main biochemical characteristics, as well as mRNA expression in the wing disc and likely protein subcellular localization for a subset of these genes. Finally, we analyze in more detail one protein family of cytoskeleton genes (Arp2/3 complex), and define the consequences of interfering with cell division for wing growth and patterning.
细胞分裂和细胞骨架组织是参与果蝇胚胎盘发育的基本过程。在本手稿中，我们描述了通过敲低可能与细胞分裂和细胞骨架组织调控相关的 85%的果蝇基因编码蛋白所导致的成年果蝇翅膀的表型。我们还将这些蛋白分子分类为描述其预期或已知主要生化特征的类别，以及这些基因中一部分的 mRNA 在翅膀盘上的表达和可能的蛋白质亚细胞定位。最后，我们更详细地分析了一个细胞骨架基因（Arp2/3 复合物）家族，并定义了干扰细胞分裂对翅膀生长和模式形成的影响。
