Centro de Biología Molecular "Severo Ochoa," CSIC and Universidad Autónoma de Madrid, Madrid 28049, Spain.
G3 (Bethesda). 2021 Dec 8;11(12). doi: 10.1093/g3journal/jkab351.
We have screened a collection of UAS-RNAi lines targeting 10,920 Drosophila protein-coding genes for phenotypes in the adult wing. We identified 3653 genes (33%) whose knockdown causes either larval/pupal lethality or a mutant phenotype affecting the formation of a normal wing. The most frequent phenotypes consist of changes in wing size, vein differentiation, and patterning, defects in the wing margin and in the apposition of the dorsal and ventral wing surfaces. We also defined 16 functional categories encompassing the most relevant aspect of each protein function and assigned each Drosophila gene to one of these functional groups. This allowed us to identify which mutant phenotypes are enriched within each functional group. Finally, we used previously published gene expression datasets to determine which genes are or are not expressed in the wing disc. Integrating expression, phenotypic and molecular information offers considerable precision to identify the relevant genes affecting wing formation and the biological processes regulated by them.
我们筛选了一组针对 10920 个果蝇蛋白编码基因的 UAS-RNAi 品系，以研究成年翅膀的表型。我们鉴定出 3653 个基因（33%），其敲低导致幼虫/蛹致死或影响正常翅膀形成的突变表型。最常见的表型包括翅膀大小、脉管分化和模式的变化、翅膀边缘和背腹翅表面的并置缺陷。我们还定义了 16 个功能类别，涵盖了每个蛋白质功能的最相关方面，并将每个果蝇基因分配到这些功能类别之一。这使我们能够确定每个功能组中哪些突变表型更为丰富。最后，我们使用以前发表的基因表达数据集来确定哪些基因在翅盘中表达或不表达。整合表达、表型和分子信息，可以非常精确地识别出影响翅膀形成的相关基因以及受它们调控的生物学过程。
