Department of Microbial Drugs, Helmholtz Centre for Infection Research and German Centre for Infection Research (DZIF), Partner Site Hannover/Braunschweig, Inhoffenstraße 7, 38124 Braunschweig, Germany.
Department of Cell Biology, Helmholtz Centre for Infection Research (HZI), Inhoffenstraße 7, 38124 Braunschweig, Germany.
Mycologia. 2023 May-Jun;115(3):277-287. doi: 10.1080/00275514.2023.2188868. Epub 2023 Apr 5.
The recent description of the putative fungal pathogen of greenheart trees, (Xylariaceae, Ascomycota), prompted a study of its secondary metabolism to access its ability to produce cytochalasans in culture. Solid-state fermentation of the ex-type strain on rice medium resulted in the isolation of a series of 19,20-epoxidated cytochalasins by means of preparative high-performance liquid chromatography (HPLC). Nine out of 10 compounds could be assigned to previously described structures, with one compound being new to science after structural assignment via nuclear magnetic resonance (NMR) assisted by high-resolution mass spectrometry (HRMS). We propose the trivial name "karyochalasin" for the unprecedented metabolite. The compounds were used in our ongoing screening campaign to study the structure-activity relationship of this family of compounds. This was done by examining their cytotoxicity against eukaryotic cells and impact on the organization of networks built by their main target, actin-a protein indispensable for processes mediating cellular shape changes and movement. Moreover, the cytochalasins' ability to inhibit the biofilm formation of and was examined.
最近对绿心树（罗汉松科，子囊菌门）潜在真菌病原体的描述促使我们对其进行了次生代谢产物的研究，以评估其在培养中产生细胞松弛素的能力。通过在大米培养基上对模式菌株进行固态发酵，利用制备高效液相色谱（HPLC）分离得到了一系列 19,20-环氧化细胞松弛素。通过高分辨率质谱（HRMS）辅助的核磁共振（NMR）对 10 种化合物中的 9 种进行了结构鉴定，其中一种化合物经结构鉴定后被认为是一种新的天然产物。我们提出了“核酮素”这个通俗名称来命名这种前所未有的代谢产物。这些化合物被用于我们正在进行的筛选研究，以研究该化合物家族的结构-活性关系。通过测试它们对真核细胞的细胞毒性以及对其主要靶标肌动蛋白（actin）所形成网络的组织的影响来进行研究。actin 是一种对于介导细胞形状变化和运动的过程必不可少的蛋白质。此外，还检测了细胞松弛素抑制 和 的生物膜形成的能力。
