Pharmaceutical Analysis Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
Tuberculosis and Lung Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Department of Anesthesiology and Intensive Care, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
J Pharm Biomed Anal. 2023 May 30;229:115377. doi: 10.1016/j.jpba.2023.115377. Epub 2023 Mar 31.
A simple and eco-friendly colorimetric sensing method has been developed for the extremely effective detection of propofol in exhaled breath condensate (EBC). In this study, we put forward a Tollens' procedure, in which silver nanoparticles (AgNPs) were produced using propofol as a reducing agent. To verify the in-situ synthesis of AgNPs, the TEM images, and UV-Vis absorbance were recorded in the absence and presence of propofol. The solution turned from a colorless to yellow and deep yellow color due to the surface plasmon resonance absorption band of formed AgNPs. The intensity of nanoparticle absorbance was quantitatively correlated with the propofol concentration. The proposed sensor revealed good linearity over the range of 0.01-0.8 µg mL at 422 nm with the detection limit of 8.8 ng mL under optimum conditions. Finally, the proposed colorimetric sensor was successfully used for the determination of propofol in the EBC sample of patients receiving propofol.
一种简单且环保的比色传感方法已被开发出来，用于对呼气冷凝物（EBC）中的异丙酚进行极其有效的检测。在本研究中，我们提出了一种Tollens 程序，其中使用异丙酚作为还原剂来制备银纳米粒子（AgNPs）。为了验证 AgNPs 的原位合成，在有无异丙酚的情况下记录了 TEM 图像和紫外-可见吸收。由于形成的 AgNPs 的表面等离子体共振吸收带，溶液从无色变为黄色和深黄色。纳米粒子吸光度的强度与异丙酚浓度呈定量相关性。在最佳条件下，该传感器在 422nm 处显示出 0.01-0.8μg mL 范围内的良好线性关系，检测限为 8.8ng mL。最后，该比色传感器成功用于检测接受异丙酚的患者 EBC 样品中的异丙酚。
