Bahcelievler State Hospital, Department of General Surgery, Istanbul, Turkey.
Department of General Surgery, Firat University, School of Medicine, Elazig, Turkey.
Pathol Res Pract. 2023 May;245:154432. doi: 10.1016/j.prp.2023.154432. Epub 2023 Mar 31.
In this study, we aimed to investigate the immunoreactivity of asprosin, irisin, and meteorin-like protein (METRNL) in different stages of colorectal adenocarcinoma, which is the most common malignancy of the gastrointestinal tract.
Overall, 60 patients with colorectal adenocarcinoma, including 20 well (Group 1), moderately (Group 2), and poorly differentiated (Group 3) cases, respectively, and 20 with normal colonic mucosa, were examined using light microscopy for immunohistochemical staining of asprosin, METRNL, and irisin.
Compared with the control group, a significant increase in irisin and asprosin immunoreactivity was found in the grade 1 and 2 colorectal adenocarcinoma groups. Moreover, compared with the grade 1 and 2 groups, this immunoreactivity was significantly decreased in the grade 3 colorectal adenocarcinoma group. Although there was no significant difference in METRNL immunoreactivity between the grade 1 and control groups, a statistically significant increase in this immunoreactivity was found in the grade 2 group. In contrast, METRNL immunoreactivity was significantly decreased in the grade 3 group compared with the grade 2 group.
We found that in early-stage colorectal adenocarcinoma there was an increase in the immunoreactivity of asprosin and irisin, but in the advanced stage there was a decrease in immunoreactivity. Although METRNL immunoreactivity did not change in the control and grade 1 groups, it was found to increase significantly in the grade 2 group and decrease in the grade 3 group.
本研究旨在探讨天冬酰胺酶、鸢尾素和 Meteorin 样蛋白（METRNL）在结直肠腺癌不同阶段的免疫反应性，结直肠腺癌是最常见的胃肠道恶性肿瘤。
共 60 例结直肠腺癌患者，包括 20 例分化良好（1 组）、中等（2 组）和低分化（3 组）病例，以及 20 例正常结肠黏膜患者，采用免疫组织化学染色法检测天冬酰胺酶、METRNL 和鸢尾素的表达。
与对照组相比，1 级和 2 级结直肠腺癌组的鸢尾素和天冬酰胺酶免疫反应性明显增加。此外，与 1 级和 2 级组相比，3 级结直肠腺癌组的这种免疫反应性显著降低。尽管 1 级组与对照组之间 METRNL 免疫反应性无显著差异，但 2 级组的 METRNL 免疫反应性显著增加。相反，与 2 级组相比，3 级组 METRNL 免疫反应性显著降低。
我们发现，在早期结直肠腺癌中，天冬酰胺酶和鸢尾素的免疫反应性增加，但在晚期则降低。虽然 METRNL 免疫反应性在对照组和 1 级组中没有变化，但在 2 级组中发现其显著增加，在 3 级组中则降低。
