Department of Pharmaceutical Sciences, Assam Central University, Silchar, Assam 788011, India.
Department of Pharmaceutical Sciences, Guru Ghasidas University, Koni, Bilaspur,(C.G 495009, India.
Biochim Biophys Acta Gen Subj. 2023 Jun;1867(6):130361. doi: 10.1016/j.bbagen.2023.130361. Epub 2023 Apr 3.
Breast cancer is the most common ailment among women. In 2020, it had the highest incidence of any type of cancer. Many Phase II and III anti-cancer drugs fail due to efficacy, durability, and side effects. Thus, accelerated drug screening models must be accurate. In-vivo models have been used for a long time, but delays, inconsistent results, and a greater sense of responsibility among scientists toward wildlife have led to the search for in-vitro alternatives. Stromal components support breast cancer growth and survival. Multi-compartment Transwell models may be handy instruments. Co-culturing breast cancer cells with endothelium and fibroblasts improves modelling. The extracellular matrix (ECM) supports native 3D hydrogels in natural and polymeric forms. 3D Transwell cultured tumor spheroids mimicked in-vivo pathological conditions. Tumor invasion, migration, Trans-endothelial migration, angiogenesis, and spread are studied using comprehensive models. Transwell models can create a cancer niche and conduct high-throughput drug screening, promising future applications. Our comprehensive shows how 3D in-vitro multi compartmental models may be useful in producing breast cancer stroma in Transwell culture.
乳腺癌是女性最常见的疾病。2020 年，它是所有癌症中发病率最高的。许多二期和三期抗癌药物由于疗效、耐久性和副作用而失败。因此，必须建立准确的加速药物筛选模型。体内模型已经使用了很长时间，但由于延迟、结果不一致以及科学家对野生动物的责任感增强，人们一直在寻找体外替代物。基质成分支持乳腺癌的生长和存活。多腔室 Transwell 模型可能是有用的工具。将乳腺癌细胞与内皮细胞和成纤维细胞共培养可改善模型。细胞外基质 (ECM) 以天然和聚合物形式支持天然 3D 水凝胶。3D Transwell 培养的肿瘤球体模拟了体内病理条件。使用综合模型研究肿瘤侵袭、迁移、跨内皮迁移、血管生成和扩散。Transwell 模型可以创建癌症生态位并进行高通量药物筛选，具有广阔的应用前景。我们的综述展示了 3D 体外多腔室模型如何在 Transwell 培养中产生乳腺癌基质方面具有应用潜力。
