Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, P.O. Box: 14665-1157, Iran.
J Mol Evol. 2023 Aug;91(4):562-566. doi: 10.1007/s00239-023-10104-5. Epub 2023 Apr 5.
About 90% of people infected with Human T lymphotropic virus type-1 (HTLV-1) virus are asymptomatic, so it can be said that the prevalence of this virus is not completely clear. During chronic infection, the expression of programmed cell death-1 (PD-1) protein increases and causes exhausted phenotype in T cells. Considering the role of host genetics and immune responses in HTLV-1 infection, in this case-control study, included 81 asymptomatic carriers (ACs) and 162 healthy controls (HCs), rs11568821 and rs41386349 polymorphisms of PD-1 gene were evaluated by Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method which investigated by one primer pair for both polymorphisms also, proviral load (PVL) measured by quantitative real-time PCR (Q-RT-PCR). The results showed that the mutant allele of rs11568821 (A) and rs41386349 (T) polymorphisms is associated with an increase in HTLV-1 infection significantly (p = 0.019 and p = 0.000 respectively). But there was no significant relationship between PVL and polymorphisms.
约 90%感染人类 T 淋巴细胞白血病病毒 1 型（HTLV-1）的人无症状，因此可以说这种病毒的流行情况并不完全清楚。在慢性感染期间，程序性细胞死亡-1（PD-1）蛋白的表达增加，导致 T 细胞出现衰竭表型。鉴于宿主遗传和免疫反应在 HTLV-1 感染中的作用，在这项病例对照研究中，纳入了 81 名无症状携带者（AC）和 162 名健康对照（HC），通过聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法评估了 PD-1 基因的 rs11568821 和 rs41386349 多态性，该方法使用一对引物同时检测两种多态性，还通过实时定量 PCR（Q-RT-PCR）测量前病毒载量（PVL）。结果表明，rs11568821（A）和 rs41386349（T）多态性的突变等位基因与 HTLV-1 感染的显著增加相关（p=0.019 和 p=0.000 分别）。但 PVL 与多态性之间没有显著关系。
