利用一条引物和前病毒载量分析 HTLV-1 感染中的程序性细胞死亡受体-1（PD-1）基因变异（re11568821 和 rs41386349）
Analysis of Programmed Cell Death-1 (PD-1) Gene Variations (re11568821 and rs41386349) in HTLV-1 Infection Using One Primer Pair and Proviral Load.
作者信息
Hezave Yalda Amiri, Sharifi Zohreh, Kermani Fahime Ranjbar
机构信息
Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, P.O. Box: 14665-1157, Iran.
出版信息
J Mol Evol. 2023 Aug;91(4):562-566. doi: 10.1007/s00239-023-10104-5. Epub 2023 Apr 5.
About 90% of people infected with Human T lymphotropic virus type-1 (HTLV-1) virus are asymptomatic, so it can be said that the prevalence of this virus is not completely clear. During chronic infection, the expression of programmed cell death-1 (PD-1) protein increases and causes exhausted phenotype in T cells. Considering the role of host genetics and immune responses in HTLV-1 infection, in this case-control study, included 81 asymptomatic carriers (ACs) and 162 healthy controls (HCs), rs11568821 and rs41386349 polymorphisms of PD-1 gene were evaluated by Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method which investigated by one primer pair for both polymorphisms also, proviral load (PVL) measured by quantitative real-time PCR (Q-RT-PCR). The results showed that the mutant allele of rs11568821 (A) and rs41386349 (T) polymorphisms is associated with an increase in HTLV-1 infection significantly (p = 0.019 and p = 0.000 respectively). But there was no significant relationship between PVL and polymorphisms.
约 90%感染人类 T 淋巴细胞白血病病毒 1 型（HTLV-1）的人无症状，因此可以说这种病毒的流行情况并不完全清楚。在慢性感染期间，程序性细胞死亡-1（PD-1）蛋白的表达增加，导致 T 细胞出现衰竭表型。鉴于宿主遗传和免疫反应在 HTLV-1 感染中的作用，在这项病例对照研究中，纳入了 81 名无症状携带者（AC）和 162 名健康对照（HC），通过聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法评估了 PD-1 基因的 rs11568821 和 rs41386349 多态性，该方法使用一对引物同时检测两种多态性，还通过实时定量 PCR（Q-RT-PCR）测量前病毒载量（PVL）。结果表明，rs11568821（A）和 rs41386349（T）多态性的突变等位基因与 HTLV-1 感染的显著增加相关（p=0.019 和 p=0.000 分别）。但 PVL 与多态性之间没有显著关系。
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