Post-graduate Program in Biophotonics Applied to Health Sciences Universidade Nove de Julho, São Paulo, Brazil.
Department of Biomedical Engineering, UFABC, São Paulo, Brazil.
Lasers Med Sci. 2022 Apr;37(3):1583-1592. doi: 10.1007/s10103-021-03407-w. Epub 2021 Nov 12.
A bone scaffold added to the dental alveolus immediately after an extraction avoids bone atrophy and deformity at the tooth loss site, enabling rehabilitation with implants. Photobiomodulation accelerates bone healing by stimulating blood flow, activating osteoblasts, diminishing osteoclastic activity, and improving the integration of the biomaterial with the bone tissue. The aim of the present study was to evaluate the effect of photobiomodulation with LED at a wavelength of 850 nm on bone quality in Wistar rats submitted to molar extraction with and without a bone graft using hydroxyapatite biomaterial (Straumann® Cerabone®). Forty-eight rats were distributed among five groups (n = 12): basal (no interventions); control (extraction) (basal and control were the same animal, but at different sides); LED (extraction + LED λ = 850 nm); biomaterial (extraction + biomaterial), and biomaterial + LED (extraction + biomaterial + LED λ = 850 nm). Euthanasia occurred at 15 and 30 days after the induction of the extraction. The ALP analysis revealed an improvement in bone formation in the control and biomaterial + LED groups at 15 days (p = 0.0086 and p = 0.0379, Bonferroni). Moreover, the LED group had better bone formation compared to the other groups at 30 days (p = 0.0007, Bonferroni). In the analysis of AcP, all groups had less resorption compared to the basal group. Bone volume increased in the biomaterial, biomaterial + LED, and basal groups in comparison to the control group at 15 days (p < 0.05, t-test). At 30 days, the basal group had greater volume compared to the control and LED groups (p < 0.05, t-test). LED combined with the biomaterial improved bone formation in the histological analysis and diminished bone degeneration (demonstrated by the reduction in AcP), promoting an increase in bone density and volume. LED may be an important therapy to combine with biomaterials to promote bone formation, along with the other known benefits of this therapy, such as the control of pain and the inflammatory process.
在拔牙后立即向牙槽骨中添加骨支架可避免牙齿缺失部位的骨萎缩和畸形，从而实现植入物的修复。光生物调节通过刺激血流、激活成骨细胞、减少破骨细胞活性以及改善生物材料与骨组织的整合来加速骨愈合。本研究的目的是评估波长为 850nm 的 LED 光生物调节对接受羟基磷灰石生物材料（Straumann® Cerabone®）种植体的 Wistar 大鼠磨牙拔除后骨质量的影响，有无骨移植。将 48 只大鼠分为五组（n=12）：基础组（无干预）；对照组（拔牙）（基础组和对照组为同一动物，但在不同侧）；LED 组（拔牙+LED λ=850nm）；生物材料组（拔牙+生物材料）和生物材料+LED 组（拔牙+生物材料+LED λ=850nm）。在拔牙诱导后 15 天和 30 天进行安乐死。碱性磷酸酶分析显示，对照组和生物材料+LED 组在 15 天时骨形成有改善（p=0.0086 和 p=0.0379，Bonferroni）。此外，LED 组在 30 天时的骨形成优于其他组（p=0.0007，Bonferroni）。在酸性磷酸酶分析中，与基础组相比，所有组的吸收都减少了。与对照组相比，在 15 天时，生物材料组、生物材料+LED 组和基础组的骨体积增加（p<0.05，t 检验）。在 30 天时，基础组的体积大于对照组和 LED 组（p<0.05，t 检验）。LED 联合生物材料可改善组织学分析中的骨形成并减少骨退化（通过降低酸性磷酸酶来证明），从而增加骨密度和体积。LED 可能是一种重要的治疗方法，可与生物材料结合以促进骨形成，同时发挥这种治疗方法的其他已知益处，例如控制疼痛和炎症过程。
