Esquivel Brooke D, White Theodore C
Department of Cell and Molecular Biology and Biochemistry, School of Biological and Chemical Sciences, University of Missouri at Kansas City, Kansas City, MO, USA.
Methods Mol Biol. 2023;2658:215-221. doi: 10.1007/978-1-0716-3155-3_15.
The ability of many antifungal molecules to traverse the fungal cell wall and accumulate within the cell is crucial to its ability to have the desired biological activity. Altered accumulation of the drug is an important mechanism of antifungal drug resistance. The best characterized mechanism for altered accumulation is through the action of the drug efflux pump which actively transports the drugs out of the membrane, although this is not the only mechanism for this phenomenon. Here, we describe protocols for the measurement of uptake of tritiated fluconazole in both yeast and filamentous fungi.
许多抗真菌分子穿越真菌细胞壁并在细胞内蓄积的能力对其产生预期生物活性的能力至关重要。药物蓄积的改变是抗真菌药物耐药性的一个重要机制。最具特征的蓄积改变机制是通过药物外排泵的作用，该泵将药物主动转运出细胞膜，尽管这不是这种现象的唯一机制。在此，我们描述了测量酵母和丝状真菌中氚标记氟康唑摄取的方法。
