Department of Cell and Molecular Biology and Biochemistry, School of Biological and Chemical Sciences, University of Missouri at Kansas City, Kansas City, MO, USA.
Methods Mol Biol. 2023;2658:201-213. doi: 10.1007/978-1-0716-3155-3_14.
One of the most prevalent mechanisms of antifungal drug resistance is export of the molecule from the fungal cells through the action of putative efflux pumps or transporters. Drug efflux is a particularly common mechanism of resistance to azole antifungals, one of the most widely used classes of antifungal drugs. Here, we provide detailed protocols for two assays of small-molecule efflux activity: rhodamine 6G efflux and alanine-naphthylamide accumulation. Protocols applicable to both yeast and filamentous fungi are provided.
抗真菌药物耐药性的最普遍机制之一是通过推测的外排泵或转运蛋白的作用将分子从真菌细胞中排出。药物外排是对唑类抗真菌药物（最广泛使用的一类抗真菌药物之一）产生耐药性的一种特别常见的机制。在这里，我们提供了两种小分子外排活性测定的详细方案：罗丹明 6G 外排和丙氨酸-萘酰胺积累。提供了适用于酵母和丝状真菌的方案。
