Nagaraj Karuppiah, Thangamuniyandi Pilavadi, Kamalesu Subramaniam, Lokhandwala Snehal, Parekh Nikhil M, Rekha Panda Swapna, Sakthinathan Subramanian, Chiu Te-Wei, Chelladurai Karuppiah, Karthikeyan Ammasai, Kalai Selvam Iruthaya
SRICT-Institute of Science and Research, UPL University of Sustainable Technology, Block No: 402, Ankleshwar - Valia Rd, Vataria, Gujarat 393135, India.
School of Chemistry, Bharathidsan University, Palkalaiperur, Tiruchirappalli-620024, Tamil Nadu, India.
Spectrochim Acta A Mol Biomol Spectrosc. 2023 Aug 5;296:122693. doi: 10.1016/j.saa.2023.122693. Epub 2023 Apr 3.
In this study, we report a new class of metallo-surfactant assisted silver nanoparticle produced by reduction process via AgNO solution and extract of Turnera Subulata (TS) in aqueous which act as reducing and metallo-surfactant Co(ip)(CHNH) (ip = imidazo[4,5-f][1,10]phenanthroline) act as stabilizing agent. In this study the silver nanoparticles produced using Turnera Subulata extract has showed yellowish brown color formation and an absorption peak at 421 nm signaling the biosynthesis of silver nanoparticles. The presence of functional groups in the plant extracts were identified by FTIR analysis. In addition, the effects of ratio, changing the concentration of the metallo surfactant, TS plant leave extract, metal precursors, and pH of the medium have been investigated on the scale of the Ag nanoparticles. Spherical shaped, crystalline in nature and ∼50 nm sized particles were recorded using TEM and DLS analysis. Furthermore, the mechanistic insights into cysteine and dopa detection by silver nanoparticles were investigated using HR-TEM analysis. This induces aggregation in stable silver nanoparticles owing to selective and strong interaction of -SH group of cysteine with silver nanoparticle surface. The biogenic Ag NPs are found to be highly sensitive to amino acids of dopa and cysteine with the diagnosis maximum for both amino acids as low as 0.9 µM (dopa) and 1 µM (cysteine) under optimized conditions.
在本研究中，我们报告了一类新型的金属表面活性剂辅助银纳米颗粒，其通过硝酸银溶液和水中的小花时钟花（TS）提取物的还原过程制备，TS提取物充当还原剂和金属表面活性剂，Co(ip)(CHNH)（ip = 咪唑并[4,5-f][1,10]菲咯啉）充当稳定剂。在本研究中，使用小花时钟花提取物制备的银纳米颗粒呈现出黄棕色，并在421nm处有一个吸收峰，表明银纳米颗粒的生物合成。通过傅里叶变换红外光谱（FTIR）分析确定了植物提取物中官能团的存在。此外，还研究了比例、改变金属表面活性剂浓度、TS植物叶提取物、金属前体和介质pH对银纳米颗粒尺寸的影响。使用透射电子显微镜（TEM）和动态光散射（DLS）分析记录了尺寸约为50nm、呈球形且为晶体的颗粒。此外，使用高分辨率透射电子显微镜（HR-TEM）分析研究了银纳米颗粒对半胱氨酸和多巴检测的机理见解。由于半胱氨酸的-SH基团与银纳米颗粒表面的选择性强相互作用，这会导致稳定的银纳米颗粒发生聚集。发现生物合成的银纳米颗粒对多巴和半胱氨酸氨基酸高度敏感，在优化条件下，两种氨基酸的诊断最大值低至0.9µM（多巴）和1µM（半胱氨酸）。
