Kalikinskaya E I, Kolomiets O L, Shevchenko V A, Bogdanov Y F
Mutat Res. 1986 May;174(1):59-65. doi: 10.1016/0165-7992(86)90077-1.
Possible implications of surface-spread synaptonemal complex (SC) karyotyping in analysing the causes of sterility of F1 from irradiated male mice are demonstrated in this work. After irradiation by 137Cs gamma-rays at a dose of 5 Gy the males were mated to unirradiated females and genetic analysis of fertility in the F1 progeny was carried out. Males with abnormal fertility were examined for the presence of chromosome aberrations in diakinesis-metaphase I and in pachytene by the method of surface-spread SC karyotyping. In most cases, SC karyotyping provides additional information and permits the detection and analysis of aberrations that are not revealed in diakinesis. Two reciprocal translocations, one X autosomal and one nonreciprocal translocation were discovered in five F1 males studied. It is concluded that the method is efficient in detecting translocations in pachytene in partially fertile F1 hybrids of irradiated and normal mice.
本文展示了表面铺展联会复合体（SC）核型分析在探究受辐照雄性小鼠F1代不育原因中的潜在意义。雄性小鼠经剂量为5 Gy的137Csγ射线辐照后，与未辐照的雌性交配，并对F1代子代的生育力进行遗传分析。通过表面铺展SC核型分析方法，对生育力异常的雄性小鼠在终变期-中期I和粗线期的染色体畸变情况进行检测。在大多数情况下，SC核型分析能提供额外信息，并可检测和分析在终变期未发现的畸变。在所研究的5只F1代雄性小鼠中发现了两个相互易位、一个X-常染色体易位和一个非相互易位。得出的结论是，该方法在检测辐照小鼠与正常小鼠的部分可育F1代杂种粗线期易位方面是有效的。
