Qu Xiaoqing, Gao Chenxi, Fu Lei, Chu Yuefeng, Wang Jian-Hua, Qiu Hongdeng, Chen Jia
Department of Chemistry, College of Sciences, Northeastern University, Shenyang 110819, China.
CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China.
ACS Appl Mater Interfaces. 2023 Apr 19;15(15):18608-18619. doi: 10.1021/acsami.2c21839. Epub 2023 Apr 9.
Bacterial infection and excess reactive oxygen species are key factors that lead to slow or substantially delayed wound healing. It is crucial to design and develop new nanomaterials with antibacterial and antioxidative capabilities for wound healing. Here, positively charged carbon dots (CDs) are rationally designed and synthesized from -phenylenediamine and polyethyleneimine by a facile one-pot solvothermal method, which show good biocompatibility in in vitro cytotoxicity, hemolysis assays, and in vivo toxicity evaluation. The positively charged CDs show superior antimicrobial effect against () at very low concentrations, reducing the risk of wound infection. At the same time, CDs with surface defects and unpaired electrons can effectively scavenge excess free radicals to reduce oxidative stress damage, accelerate wound inflammation-proliferation transition, and promote wound healing. The mouse model of skin infection demonstrates that CDs can effectively promote the wound healing of skin infection without obvious side effects by simply dropping or spraying onto the wound. We believe that the prepared CDs have satisfactory biocompatibility, antioxidant capacity, and excellent antibacterial activity and have great application potential in wound healing.
细菌感染和过量的活性氧是导致伤口愈合缓慢或显著延迟的关键因素。设计和开发具有抗菌和抗氧化能力的新型纳米材料对于伤口愈合至关重要。在此，通过简便的一锅溶剂热法，由对苯二胺和聚乙烯亚胺合理设计并合成了带正电荷的碳点（CDs），其在体外细胞毒性、溶血试验和体内毒性评估中表现出良好的生物相容性。带正电荷的CDs在极低浓度下对（）显示出优异的抗菌效果，降低了伤口感染的风险。同时，具有表面缺陷和未成对电子的CDs可以有效清除过量的自由基，减少氧化应激损伤，加速伤口炎症-增殖转变，促进伤口愈合。皮肤感染小鼠模型表明，通过简单地滴加或喷洒到伤口上，CDs可以有效促进皮肤感染伤口的愈合且无明显副作用。我们认为，所制备的CDs具有令人满意的生物相容性、抗氧化能力和优异的抗菌活性，在伤口愈合方面具有巨大的应用潜力。
