-毒性突变体的转录组分析为……的……提供了线索。（注：原文部分内容缺失，翻译可能不太完整准确）

Transcriptome analysis of -virulent mutants provides clues for the of .

作者信息

Cui Zhongchi, Wu Wenyue, Fan Fan, Wang Fei, Liu Daqun, Di Dianping, Wang Haiyan

机构信息

College of Plant Protection, Technological Innovation Center for Biological Control of Crop Diseases and Insect Pests of Hebei Province, Hebei Agricultural University, Baoding, Hebei, China.

Plant Protection Institute, Hebei Academy of Agricultural and Forestry Sciences, Baoding, Hebei, China.

出版信息

Front Microbiol. 2023 Mar 22;14:1062548. doi: 10.3389/fmicb.2023.1062548. eCollection 2023.

DOI:10.3389/fmicb.2023.1062548

PMID:37032911

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10073493/

Abstract

INTRODUCTION

Wheat leaf rust caused by () remains one of the most destructive diseases of common wheat worldwide. Understanding the pathogenicity mechanisms of is important to control wheat leaf rust.

METHODS

The urediniospores of race PHNT (wheat leaf rust resistance gene -avirulent isolate) were mutagenized with ethyl methanesulfonate (EMS), and two -virulent mutants named M1 and M2 were isolated. RNA sequencing was performed on samples collected from wheat cultivars Chinese Spring and TcLr19 infected with wild-type (WT) PHNT, M1, and M2 isolates at 14 days post-inoculation (dpi), respectively. Screening candidates by quantitative reverse transcription PCR (qPCR) and Agrobacterium-mediated transient assays in .

RESULTS

560 genes with single nucleotide polymorphisms (SNPs) and insertions or deletions (Indels) from non-differentially expressed genes were identified. Among them, 10 secreted proteins were screened based on their fragments per kilobase of exon model per million mapped reads (FPKM) values in the database. qPCR results showed that the expression profiles of 7 secreted proteins including PTTG_27471, PTTG_12441, PTTG_28324, PTTG_26499, PTTG_06910, PTTG_26516, and PTTG_03570 among 10 secreted proteins in mutants were significantly different with that in wild-type isolate after infection wheat TcLr19 and might be related to the recognition between and . In addition, a total of 216 differentially expressed genes (DEGs) were obtained from three different sample comparisons including M1-vs-WT, M2-vs-WT, and M1-vs-M2. Among 216 DEGs, 15 were predicted to be secreted proteins. One secreted protein named PTTG_04779 could inhibit programmed progress of cell death (PCD) induced by apoptosis-controlling genes B-cell lymphoma-2 associated X protein (BAX) on , indicating that it might play a virulence function in plant. Taken together, total 8 secreted proteins, PTTG_04779, PTTG_27471, PTTG_12441, PTTG_28324, PTTG_26499, PTTG_06910, PTTG_26516, PTTG_03570 are identified as candidates.

DISCUSSION

Our results showed that a large number of genes participate in the interaction between and TcLr19, which will provide valuable resources for the identification of candidates and pathogenesis-related genes.

摘要

引言

由（）引起的小麦叶锈病仍是全球普通小麦最具毁灭性的病害之一。了解（）的致病机制对于防治小麦叶锈病至关重要。

方法

用甲基磺酸乙酯（EMS）诱变小麦叶锈菌生理小种PHNT（对小麦叶锈病抗性基因无毒力的分离株）的夏孢子，分离出两个有毒力的突变体，命名为M1和M2。分别在接种后14天（dpi），对感染野生型（WT）PHNT、M1和M2分离株的小麦品种中国春和TcLr19采集的样本进行RNA测序。通过定量逆转录PCR（qPCR）和农杆菌介导的瞬时分析在（）中筛选（）候选基因。

结果

从非差异表达基因中鉴定出560个具有单核苷酸多态性（SNP）和插入或缺失（Indel）的基因。其中，根据数据库中每百万映射读数中每千碱基外显子模型片段数（FPKM）值筛选出10个分泌蛋白。qPCR结果表明，在感染小麦TcLr19后，10个分泌蛋白中的7个分泌蛋白（包括PTTG_27471、PTTG_12441、PTTG_28324、PTTG_26499、PTTG_06910、PTTG_26516和PTTG_03570）在突变体中的表达谱与野生型分离株中的表达谱有显著差异，可能与（）和（）之间的识别有关。此外，从包括M1与WT、M2与WT以及M1与M2的三个不同样本比较中总共获得216个差异表达基因（DEG）。在216个DEG中，有15个被预测为分泌蛋白。一个名为PTTG_04779的分泌蛋白可以抑制凋亡控制基因B细胞淋巴瘤-2相关X蛋白（BAX）在（）上诱导的细胞程序性死亡（PCD）进程，表明它可能在植物中发挥毒力功能。综上所述，总共8个分泌蛋白PTTG_04779、PTTG_27471,、PTTG_12441、PTTG_28324、PTTG_26499、PTTG_06910、PTTG_26516、PTTG_03570被鉴定为（）候选基因。