Zheng Yunxi, He Reqing
Queen Mary School, Nanchang University, Nanchang 330031, China.
Key Laboratory of Molecular Biology and Gene Engineering in Jiangxi Province, College of Life Science, Nanchang University,Nanchang 330031, China.
MicroPubl Biol. 2023 Mar 20;2023. doi: 10.17912/micropub.biology.000814. eCollection 2023.
DNA methylation is a stable and heritable epigenetic mark, and it plays an important role in regulation of gene expression and transposon silencing. Here we developed a CRISPR/dCpf1-based targeted demethylation system using the catalytic domain of the human demethylase TEN-ELEVEN TRANSLOCATION1 (TET1cd) and a SunTag system. The SunTag-dCpf1-TET1cd system is able to achieve targeted DNA demethylation and up-regulate gene expression when guided to the or loci in . Our study provides tools for targeted removal of DNA cytosine methylation, and activation of protein-coding genes or transposons expression.
DNA甲基化是一种稳定且可遗传的表观遗传标记，它在基因表达调控和转座子沉默中发挥着重要作用。在此，我们利用人类去甲基化酶TEN-ELEVEN TRANSLOCATION1（TET1cd）的催化结构域和SunTag系统，开发了一种基于CRISPR/dCpf1的靶向去甲基化系统。当被引导至人细胞的特定基因座时，SunTag-dCpf1-TET1cd系统能够实现靶向DNA去甲基化并上调基因表达。我们的研究为靶向去除DNA胞嘧啶甲基化以及激活蛋白质编码基因或转座子表达提供了工具。
