Mishra Dwijesh Chandra, Majumdar Sayanti Guha, Kumar Anuj, Bhati Jyotika, Chaturvedi K K, Kumar Ranjeet Ranjan, Goswami Suneha, Rai Anil, Budhlakoti Neeraj
ICAR-Indian Agricultural Statistics Research Institute, New Delhi, India.
ICAR-Indian Agricultural Research Institute, New Delhi, India.
Int J Genomics. 2023 Mar 30;2023:1774764. doi: 10.1155/2023/1774764. eCollection 2023.
Climate change has become a major source of concern, particularly in agriculture, because it has a significant impact on the production of economically important crops such as wheat, rice, and maize. In the present study, an attempt has been made to identify differentially expressed heat stress-responsive long non-coding RNAs (lncRNAs) in the wheat genome using publicly available wheat transcriptome data (24 SRAs) representing two conditions, namely, control and heat-stressed. A total of 10,965 lncRNAs have been identified and, among them, 153, 143, and 211 differentially expressed transcripts have been found under 0 DAT, 1 DAT, and 4 DAT heat-stress conditions, respectively. Target prediction analysis revealed that 4098 lncRNAs were targeted by 119 different miRNA responses to a plethora of environmental stresses, including heat stress. A total of 171 hub genes had 204 SSRs (simple sequence repeats), and a set of target sequences had SNP potential as well. Furthermore, gene ontology analysis revealed that the majority of the discovered lncRNAs are engaged in a variety of cellular and biological processes related to heat stress responses. Furthermore, the modeled three-dimensional (3D) structures of hub genes encoding proteins, which had an appropriate range of similarity with solved structures, provided information on their structural roles. The current study reveals many elements of gene expression regulation in wheat under heat stress, paving the way for the development of improved climate-resilient wheat cultivars.
气候变化已成为一个主要的关注点，尤其是在农业领域，因为它对小麦、水稻和玉米等具有重要经济价值的作物生产有重大影响。在本研究中，我们尝试利用公开可用的小麦转录组数据（24个SRA）来鉴定小麦基因组中差异表达的热应激响应长链非编码RNA（lncRNA），这些数据代表了对照和热应激两种条件。总共鉴定出10965个lncRNA，其中分别在热应激0天、1天和4天的条件下发现了153、143和211个差异表达的转录本。靶标预测分析表明，4098个lncRNA被119种不同的miRNA靶向，这些miRNA对包括热应激在内的多种环境胁迫作出反应。共有171个枢纽基因具有204个简单序列重复（SSR），并且一组靶标序列也具有SNP潜力。此外，基因本体分析表明，大多数发现的lncRNA参与了与热应激反应相关的各种细胞和生物学过程。此外，编码蛋白质的枢纽基因的三维（3D）结构模型与已解析结构具有适当范围的相似性，提供了它们结构作用的信息。当前的研究揭示了热应激下小麦基因表达调控的许多要素，为培育具有更强气候适应性的小麦品种铺平了道路。
