School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, Punjab, 141004, India.
Department of Biosciences and Bioengineering, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, 247667, India.
Sci Rep. 2021 Nov 11;11(1):22049. doi: 10.1038/s41598-021-01596-6.
Wheat, one of the major cereal crops worldwide, get adversely affected by rising global temperature. We have identified the diploid B genome progenitor of wheat, Aegilops speltoides (SS), as a potential donor for heat stress tolerance. Therefore, the present work was planned to study the total transcriptome profile of heat stress-tolerant Ae. speltoides accession pau3809 (AS3809) and compare with that of tetraploid and hexaploid wheat cultivars PDW274 and PBW725, respectively. The comparative transcriptome was utilized to identify and validate heat stress transcription factors (HSFs), the key genes involved in imparting heat stress tolerance. Transcriptome analysis led to the identification of a total of 74 K, 68 K, and 76 K genes in AS3809, PDW274, and PBW725, respectively. There was a high uniformity of GO profiles under the biological, molecular, and cellular functions across the three wheat transcriptomes, suggesting the conservation of gene function. Twelve HSFs having the highest FPKM value were identified in the AS3809 transcriptome data, while six of these HSFs namely HSFA3, HSFA5, HSFA9, HSFB2a, HSFB2b, and HSFC1b, were validated with qRT PCR. These six HSFs were identified as an important component of thermotolerance in AS3809 as evident from their comparative higher expression under heat stress.
小麦是世界上主要的谷物作物之一，受到全球气温升高的不利影响。我们已经确定了小麦的二倍体 B 基因组祖先是节节麦（SS），它是耐热的潜在供体。因此，本研究旨在研究耐热节节麦 Ae. speltoides 品系 pau3809（AS3809）的总转录组谱，并与四倍体和六倍体小麦品种 PDW274 和 PBW725 进行比较。比较转录组用于鉴定和验证参与耐热性的热激转录因子（HSFs），这是关键基因。转录组分析总共鉴定出 AS3809、PDW274 和 PBW725 中的 74K、68K 和 76K 基因。三个小麦转录组在生物、分子和细胞功能下的 GO 图谱具有很高的一致性，表明基因功能的保守性。在 AS3809 转录组数据中鉴定出了 12 个具有最高 FPKM 值的 HSFs，其中 6 个 HSFs（HSFA3、HSFA5、HSFA9、HSFB2a、HSFB2b 和 HSFC1b）通过 qRT-PCR 进行了验证。这些 6 个 HSFs 被认为是 AS3809 耐热性的重要组成部分，因为它们在热胁迫下的表达水平较高。
