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使用HMG17特异性单克隆抗体从活性染色质中分离寡核小体。

Isolation of oligonucleosomes from active chromatin using HMG17-specific monoclonal antibodies.

作者信息

Dorbic T, Wittig B

出版信息

Nucleic Acids Res. 1986 Apr 25;14(8):3363-76. doi: 10.1093/nar/14.8.3363.

DOI:10.1093/nar/14.8.3363
PMID:3703677
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC339778/
Abstract

We report the preparation of HMG17-containing oligonucleosomes from chicken embryos and from liver and oviduct of laying hens. Monoclonal antibodies against HMG17 were used for their isolation. An unusual size distribution with respect to their repeat number was observed. The oligonucleosomes of repeat number up to N6 were highly enriched for DNA of the vitellogenin II gene (liver) and for DNA of the ovalbumin and lysozyme genes (oviduct).

摘要

我们报道了从鸡胚以及产蛋母鸡的肝脏和输卵管中制备含HMG17的寡核小体。使用抗HMG17单克隆抗体对其进行分离。观察到其重复数的大小分布异常。重复数高达N6的寡核小体高度富集卵黄蛋白原II基因（肝脏）的DNA以及卵清蛋白和溶菌酶基因（输卵管）的DNA。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f4d4/339778/1c93439d0c63/nar00277-0224-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f4d4/339778/76c1e203e661/nar00277-0217-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f4d4/339778/def2d346449a/nar00277-0219-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f4d4/339778/6d8d05313dc5/nar00277-0220-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f4d4/339778/913fd2e24e9f/nar00277-0221-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f4d4/339778/1c93439d0c63/nar00277-0224-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f4d4/339778/76c1e203e661/nar00277-0217-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f4d4/339778/def2d346449a/nar00277-0219-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f4d4/339778/6d8d05313dc5/nar00277-0220-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f4d4/339778/913fd2e24e9f/nar00277-0221-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f4d4/339778/1c93439d0c63/nar00277-0224-a.jpg

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本文引用的文献

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One-step purification of mouse monoclonal antibodies from ascitic fluid by DEAE Affi-Gel blue chromatography.通过DEAE亲和蓝胶色谱法从腹水原液中一步纯化小鼠单克隆抗体。
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Chromatin from transcribed genes contains HMG17 only downstream from the starting point of transcription.来自转录基因的染色质仅在转录起始点下游含有高迁移率族蛋白17（HMG17）。
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