聚（5-甲基胞嘧啶-脱氧鸟苷）钠盐的低离子强度形式是B型DNA。
The low ionic strength form of the sodium salt of poly(dm5C-dG) is a B DNA.
作者信息
Woisard A, Guschlbauer W, Fazakerley G V
出版信息
Nucleic Acids Res. 1986 Apr 25;14(8):3515-9. doi: 10.1093/nar/14.8.3515.
Abstract
The low-salt Z form of poly(dm5C-dG) reported in the literature was shown to be due to minute amounts of bivalent cations. If the polymer was purified by dialysis against 1 mM EDTA, poly(dm5C-dG) showed the normal B form. No change in this B form was observed up to 0.8 M NaCl. Very small amounts of Mg2+ or Ca2+ (10(-6) to 10(-5) M) induced the characteristic Z form CD spectrum, which could easily and rapidly be reverted to B-DNA by addition of EDTA.
摘要
文献中报道的聚（dm5C-dG）的低盐Z型被证明是由于微量二价阳离子所致。如果通过用1 mM EDTA透析纯化该聚合物，聚（dm5C-dG）会呈现正常的B型。在高达0.8 M NaCl的情况下，未观察到这种B型有变化。极少量的Mg2+或Ca2+（10^(-6)至10^(-5) M）会诱导出特征性的Z型CD光谱，通过添加EDTA可以轻松快速地将其恢复为B-DNA。
相似文献
1
The low ionic strength form of the sodium salt of poly(dm5C-dG) is a B DNA.聚（5-甲基胞嘧啶-脱氧鸟苷）钠盐的低离子强度形式是B型DNA。
Nucleic Acids Res. 1986 Apr 25;14(8):3515-9. doi: 10.1093/nar/14.8.3515.
2
Z-DNA is formed by poly (dC-dG) and poly (dm5C-dG) at micro or nanomolar concentrations of some zinc(II) and copper(II) complexes.
J Biomol Struct Dyn. 1985 Jun;2(6):1205-20. doi: 10.1080/07391102.1985.10507633.
3
Effect of template conversion from the B to the Z conformation on RNA polymerase activity.模板从B构象转变为Z构象对RNA聚合酶活性的影响。
Biochemistry. 1984 Oct 9;23(21):4837-43. doi: 10.1021/bi00316a004.
4
New DNA polymorphism: evidence for a low salt, left-handed form of poly(dG-m5dC).新的DNA多态性：聚（dG-m5dC）低盐、左手形式的证据。
Nucleic Acids Res. 1985 Jun 11;13(11):4133-41. doi: 10.1093/nar/13.11.4133.
5
Toroidal condensation of Z DNA and identification of an intermediate in the B to Z transition of poly(dG-m5dC) X poly(dG-m5dC).Z-DNA的环形凝聚以及聚（dG-m5dC）×聚（dG-m5dC）从B型到Z型转变中间体的鉴定。
Biochemistry. 1985 Jan 29;24(3):713-9. doi: 10.1021/bi00324a026.
6
Z-DNA: vacuum ultraviolet circular dichroism.Z型DNA：真空紫外圆二色性
Proc Natl Acad Sci U S A. 1981 Aug;78(8):4801-4. doi: 10.1073/pnas.78.8.4801.
7
A novel structural transition in poly(dG-Me5dC):Z in equilibrium B in equilibrium Z.聚（dG-Me5dC）中一种新的结构转变：在平衡态B与平衡态Z之间。
FEBS Lett. 1985 Mar 25;182(2):315-8. doi: 10.1016/0014-5793(85)80323-9.
8
Role of divalent cations on DNA polymorphism under low ionic strength conditions.二价阳离子在低离子强度条件下对DNA多态性的作用
Nucleic Acids Res. 1986 Jun 25;14(12):5099-109. doi: 10.1093/nar/14.12.5099.
9
Intensity changes of base and backbone Raman modes in the B to Z transition of poly(dG-dm5C).
J Biomol Struct Dyn. 1986 Jun;3(6):1189-202. doi: 10.1080/07391102.1986.10508494.
10
Slow exchanging protons in the Z-form of G-C and A-C alternating polymers by using a rapid dialysis method.通过快速透析法在G-C和A-C交替聚合物的Z型中缓慢交换质子。
FEBS Lett. 1987 Dec 10;225(1-2):11-5. doi: 10.1016/0014-5793(87)81122-5.
引用本文的文献
1
Stabilization of the Z' form of poly(dGdC):poly(dGdC) in solution by multivalent ions relates to the ZII form in crystals.多价离子使溶液中的聚（dGdC）:聚（dGdC）的Z' 形式稳定，这与晶体中的ZII形式有关。
Nucleic Acids Res. 1990 Apr 25;18(8):2141-8. doi: 10.1093/nar/18.8.2141.
本文引用的文献
1
Is Tb3+ fluorescence enhancement only due to binding to single stranded polynucleotides.Tb3+荧光增强仅仅是由于与单链多核苷酸结合吗？
Nucleic Acids Res. 1981 Nov 25;9(22):6191-7. doi: 10.1093/nar/9.22.6191.
2
The B goes to Z transition of poly(dG-dC) . poly(dG-dC) modified by some platinum derivatives.聚（dG-dC）·聚（dG-dC）经某些铂衍生物修饰后的B到Z转变。
Nucleic Acids Res. 1981 Nov 11;9(21):5659-69. doi: 10.1093/nar/9.21.5659.
3
Mn2+ and other transition metals at low concentration induce the right-to-left helical transformation of poly[d(G-C)].低浓度的锰离子（Mn2+）和其他过渡金属会诱导聚[d(G-C)]从右向左的螺旋转变。
EMBO J. 1982;1(7):777-82. doi: 10.1002/j.1460-2075.1982.tb01247.x.
4
Zinc Z-DNA.锌与Z型DNA
Nucleic Acids Res. 1984 Apr 25;12(8):3643-8. doi: 10.1093/nar/12.8.3643.
5
Critical amount of oligovalent ion binding required for the B-Z transition of poly (dG-m5dC).聚（dG-m5dC）从B型向Z型转变所需的低价位离子结合的临界量。
Nucleic Acids Res. 1984 Mar 12;12(5):2381-9. doi: 10.1093/nar/12.5.2381.
6
A thermally driven interconversion of B and Z-dna.B型和Z型DNA的热驱动相互转换
Biochem Biophys Res Commun. 1983 Aug 30;115(1):100-5. doi: 10.1016/0006-291x(83)90974-9.
7
8
Effects of methylation on a synthetic polynucleotide: the B--Z transition in poly(dG-m5dC).poly(dG-m5dC).甲基化对合成多核苷酸的影响：聚（dG-m5dC）·聚（dG-m5dC）中的B-Z转变
Proc Natl Acad Sci U S A. 1981 Mar;78(3):1619-23. doi: 10.1073/pnas.78.3.1619.
9
Z* DNA, the left-handed helical form of poly[d(G-C)] in MgCl2-ethanol, is biologically active.Z型DNA，即聚[d（G-C）]在氯化镁-乙醇中的左手螺旋形式，具有生物活性。
EMBO J. 1982;1(1):115-20. doi: 10.1002/j.1460-2075.1982.tb01133.x.
10
A low-salt form of poly(dG-5M-dC).poly(dG-5M-dC).聚（dG-5M-dC）.聚（dG-5M-dC）的低盐形式
Biopolymers. 1985 May;24(5):905-10. doi: 10.1002/bip.360240513.
Zotero 插件