Haertlé T, Augustyniak J, Guschlbauer W
Nucleic Acids Res. 1981 Nov 25;9(22):6191-7. doi: 10.1093/nar/9.22.6191.
Enhancement of Tb3+ fluorescence upon binding to double-stranded ribo- and deoxyribo-duplexes was investigated. It was observed that certain double stranded ribopolynucleotides completely quenched the Tb3+ fluorescence and others did not. It is concluded that the nature of the base in the duplex is critical for this enhancement. - Polydeoxyduplexes also showed enhancement of Tb3+ fluorescence, but much higher terbium concentrations were necessary to obtain similar fluorescence signals, indicative of unspecific effects. CD spectra evidence considerable conformational changes of these duplexes, in particular poly(dG-C) . poly(dG-C( which assumes the Z-form in 0.1 nM Tb3+.
研究了Tb3+与双链核糖核酸和脱氧核糖核酸双链体结合时荧光的增强情况。观察到某些双链核糖多核苷酸完全淬灭了Tb3+的荧光，而其他的则没有。得出结论，双链体中碱基的性质对这种增强至关重要。 - 聚脱氧双链体也显示出Tb3+荧光的增强，但需要更高的铽浓度才能获得相似的荧光信号，这表明存在非特异性效应。圆二色光谱证明了这些双链体有相当大的构象变化，特别是聚（dG-C）。聚（dG-C）在0.1 nM Tb3+中呈Z型。
