Bach Institute of Biochemistry, Fundamentals of Biotechnology Federal Research Center, Russian Academy of Sciences, Moscow 119071, Russia.
Bioresour Technol. 2023 Jul;379:129030. doi: 10.1016/j.biortech.2023.129030. Epub 2023 Apr 8.
The review describes the advances in the phytase immobilization for the past decade and their biotechnological applications. Different approaches for phytase immobilization are described including the process using organic and inorganic matrices and microbial cells, as well as nanostructures of various nature. Moreover, the immobilization of phytase-producing microbial cells and the use of cross-linked phytase aggregates have been under consideration. A detailed classification of various carriers for immobilization of phytases and the possibility of their applications are presented. A particular attention is drawn to a breakthrough approach of biotechnological significance to the design of microencapsulation of bacterial phytase from Obesumbacterium proteus in the recombinant extremophile of Yarrowia lipolytica.
该综述描述了过去十年中植酸酶固定化的进展及其在生物技术中的应用。描述了不同的植酸酶固定化方法，包括使用有机和无机基质以及微生物细胞的方法，以及各种性质的纳米结构。此外，还考虑了植酸酶产生微生物细胞的固定化和交联植酸酶聚集体的使用。本文详细分类了各种用于植酸酶固定化的载体，并介绍了它们的应用可能性。特别关注了从恶臭假单胞菌中设计重组极端嗜热酵母脂肪酶微胶囊化的植酸酶的生物技术意义上的突破方法。
