Neubauer B L, Bemis K G, Best K L, Goode R L, Merriman R L, Smith G F, Tanzer L R, Hoover D M
Prostate. 1986;8(3):265-76. doi: 10.1002/pros.2990080307.
The spontaneous metastatic spread of a suspension of PAIII prostatic adenocarcinoma cells from the tail site of implantation was analyzed over a period of 5 weeks in male Lobund-Wistar (LW) rats. Following subcutaneous injection of the PAIII cells, the tumor metastasized through the primary lymphatic drainage. PAIII microfoci were evident in the gluteal and iliac lymph nodes prior to colonization of the lungs. Growth of the primary tumor was evidenced by significant weight differences of the tails of PAIII-bearing and control rats 1 week after tumor implantation. Time-dependent sequential spread of the adenocarcinoma was quantitated. Significant differences were noted between PAIII-bearing and control animals with respect to the gluteal lymph node weights (+2 weeks), iliac lymph node weights (+3 weeks), dry lung weights, and lung colony numbers (+4 weeks) after tumor implantation. During the course of these studies, the whole blood prothrombin, activated partial thromboplastin, and recalcification times for the PAIII-bearing animals were similar to those of the control group. These findings indicate that there were no gross changes in systemic blood coagulation accompanying the metastasis of PAIII cells from the primary tumor. The tumor in LW rats produced a consistent pattern of growth and metastasis that is suitable for quantitation. The PAIII prostatic adenocarcinoma is a sensitive and reproducible system that may be useful to evaluate potential antimetastatic and cytotoxic agents for the treatment of hormone-insensitive prostatic cancer.
在雄性Lobund-Wistar（LW）大鼠中，对PAIII前列腺腺癌细胞悬液从植入尾部部位的自发转移扩散情况进行了为期5周的分析。皮下注射PAIII细胞后，肿瘤通过初级淋巴引流发生转移。在肺部定植之前，臀淋巴结和髂淋巴结中可见PAIII微病灶。肿瘤植入1周后，携带PAIII的大鼠和对照大鼠尾巴重量存在显著差异，证明了原发性肿瘤的生长。对腺癌的时间依赖性顺序扩散进行了定量分析。肿瘤植入后，在臀淋巴结重量（+2周）、髂淋巴结重量（+3周）、肺干重和肺集落数（+4周）方面，携带PAIII的动物和对照动物之间存在显著差异。在这些研究过程中，携带PAIII的动物的全血凝血酶原、活化部分凝血活酶和复钙时间与对照组相似。这些发现表明，PAIII细胞从原发性肿瘤转移时，全身血液凝固没有明显变化。LW大鼠中的肿瘤产生了一致的生长和转移模式，适合进行定量分析。PAIII前列腺腺癌是一个敏感且可重复的系统，可能有助于评估治疗激素不敏感型前列腺癌的潜在抗转移和细胞毒性药物。
